Abstract
The enzymatic destruction of chitosan in an acetic acid solution in the presence of sodium chloride and sulfate was considered. It was shown that the addition of these low-molecular electrolytes is accompanied by a tightening of the macromolecular coil, as indicated by a decrease in the α constant in the Mark–Kuhn–Houwink equation and the intrinsic viscosity of chitosan. As a consequence, the chitosan units become less accessible for interaction with the enzyme, and the kinetic parameters of the process (Michaelis constant K M and the maximum rate of enzymatic destruction V max) change accordingly. This suggests that the resistance of this polymer to the enzyme action can be enhanced by introducing low-molecular electrolyte salts in the solution or film coating to suppress the polyelectrolyte swelling of chitosan.
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Original Russian Text © V.V. Chernova, I.F. Tuktarova, E.I. Kulish, 2017, published in Khimicheskaya Fizika, 2017, Vol. 36, No. 4, pp. 75–80.
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Chernova, V.V., Tuktarova, I.F. & Kulish, E.I. On the possibility of regulating the rate of enzymatic destruction of chitosan in an acetic acid solution. Russ. J. Phys. Chem. B 11, 338–342 (2017). https://doi.org/10.1134/S1990793117020166
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DOI: https://doi.org/10.1134/S1990793117020166