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Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract—

A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of the reference method, MTT-based cell viability assay.

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Funding

The work was carried out with the use of budget funds as a part of the State Assignment Registration No. 122020100109-6 at the Samara State Medical University of the Ministry of Health of Russia.

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Authors and Affiliations

  1. Samara State Medical University, Ministry of Health of the Russian Federation, 443099, Samara, Russia

    P. V. Iliasov, L. V. Limareva, A. I. Sizova & A. P. Kuricyna

  2. Samara State Technical University, 443100, Samara, Russia

    V. A. Zalomlenkov

Authors
  1. P. V. Iliasov
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  2. L. V. Limareva
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  3. A. I. Sizova
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  4. V. A. Zalomlenkov
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Correspondence to A. I. Sizova.

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The authors declare that they have no conflict of interest.

This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Sizova

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Abbreviations: 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine, 2-ChMMT.

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Iliasov, P.V., Limareva, L.V., Sizova, A.I. et al. Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes. Biochem. Moscow Suppl. Ser. A 17, 269–275 (2023). https://doi.org/10.1134/S1990747823050057

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