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Investigations of Electrogenic Ion Transport by Na+,K+-ATPase in Bilayer Lipid Membranes by Impedance Method

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract—

The results of investigation of electrogenic transport by the Na+,K+-ATPase, the enzyme providing the active transport of Na+ and K+ ions through cell membrane, are reviewed. The main contribution to electric current generated through the functioning of the Na+,K+-ATPase is assigned to the movements of ions in access channels—the channel-like structures connecting the ion binding sites with the solutions. The electrogenic transport was studied in a model system consisting of a bilayer lipid membrane with adsorbed membrane fragments containing the Na+,K+-ATPase. The impedance method applied to this study allowed the investigation of access channels in the Na+,K+-ATPase. The review notes a significant contribution of Yu.A. Chizmadzhev to the development of the theoretical model of transport processes in the Na+,K+-ATPase.

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Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation (registration number of the project, 122011300058-3).

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  1. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071, Moscow, Russia

    V. S. Sokolov

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Correspondence to V. S. Sokolov.

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Translated by E. Makeeva

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Sokolov, V.S. Investigations of Electrogenic Ion Transport by Na+,K+-ATPase in Bilayer Lipid Membranes by Impedance Method. Biochem. Moscow Suppl. Ser. A 16, 282–290 (2022). https://doi.org/10.1134/S1990747822050117

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