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Application of 3D Scaffolds in Tissue Engineering

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Abstract—

Three-dimensional (3D) scaffolds are often used in tissue engineering applications to produce an environment that is conducive to the integration of cells or growth factors to repair or replace damaged tissues or organs. These scaffolds are utilized to mimic the microenvironment seen in vivo, where cells interact and respond to mechanical cues from their three-dimensional surroundings. Consequently, cellular response and fate depend greatly on the material properties of scaffolds. These three-dimensional scaffolds' porous, networked pore structures enable the movement of nutrients, oxygen, and waste. This article looks at the many manufacturing procedures (such as conventional and rapid prototyping techniques) used to create 3D scaffolds with variable pore sizes and porosities. The various methods for determining pore size and porosity will also be covered. It has also been investigated if scaffolds with graded porosity may more accurately mimic the in vivo situation in which cells are exposed to layers of various tissues with changing characteristics. Following a look at the extracellular matrix, nature’s own scaffold, the ability of scaffold pore size and porosity to affect biological responses and mechanical qualities will also be investigated. We will talk about the problems with the current ways of building scaffolds for tissue engineering applications and offer some new and exciting alternatives.

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ACKNOWLEDGMENTS

The authors would like to thank Mohaghegh Ardabili University for concerning this manuscript.

Funding

The research was funded by University of Mohaghegh Ardabili.

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Authors and Affiliations

  1. Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Seyedeh Mahdieh Khoshnazar

  2. Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran

    Asadollah Asadi, Saman Roshancheshm & Aida Karimian

  3. Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran

    Arash Abdolmaleki

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  1. Seyedeh Mahdieh Khoshnazar
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Correspondence to Arash Abdolmaleki.

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Khoshnazar, S.M., Asadi, A., Roshancheshm, S. et al. Application of 3D Scaffolds in Tissue Engineering. Cell Tiss. Biol. 17, 454–464 (2023). https://doi.org/10.1134/S1990519X23050061

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