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Nitroxidergic and Calretinin-Containing Nonpyramidal Neurons of Rat Hippocampus

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Abstract—

Unlike pyramidal neurons, interneurons of the hippocampus are a heterogeneous group of neurons that differ in their morphological, cytochemical, and functional characteristics. The aim of this study was to conduct a comparative morphological analysis of nitroxidergic (NOS+) and calretinin-containing (CR+) nonpyramidal neurons of the CA1 and CA3 zones in the rat hippocampus using immunohistochemical methods. Qualitative and quantitative differences in NOS+ and CR+ populations of nonpyramidal neurons in different layers of the studied zones of the hippocampus were demonstrated. Differences in the quantitative composition of the studied populations of interneurons were also demonstrated in two subregions of the hippocampus that correspond to dorsal and intermediate hippocampus. It was established that NOS+ interneurons are more typical for the dorsal hippocampus, while a greater number of CR+ interneurons are presented in the intermediate hippocampus. Data obtained during the study can contribute to understanding the role of nonpyramidal neurons in the formation of functional specialization of different regions of the hippocampus.

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Funding

This work was carried out within the framework of an order to the Institute of Experimental Medicine.

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  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    I. O. Ilyasov, D. A. Sufieva & D. E. Korzhevskii

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  1. I. O. Ilyasov
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Correspondence to D. A. Sufieva.

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Statement on the welfare of animals. When working with animals, all applicable international and national principles of humane treatment of animals were followed. When keeping and killing animals, international regulations of the Helsinki Declaration on the Humane Treatment of Animals and “Rules for Conducting Work using Experimental Animals” (appendix to the order of the Ministry of Health of the Soviet Union no. 755 of August 12, 1977) were followed.

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Translated by A. Barkhash

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Ilyasov, I., Sufieva, D. & Korzhevskii, D. Nitroxidergic and Calretinin-Containing Nonpyramidal Neurons of Rat Hippocampus. Cell Tiss. Biol. 16, 478–486 (2022). https://doi.org/10.1134/S1990519X22050030

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