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Immunolocalization of BDNF, GDNF, and NT-3 in the Rat Parietal Cortex with Permanent Occlusion of the Middle Cerebral Artery

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Abstract—

Immunolocalization of brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and glial cell line-derived neurotrophic factor (GDNF) in the parietal cortex of rats in a model of focal stroke caused by permanent occlusion of the middle cerebral artery was studied. The spatial density of marked cells constantly varies by cortex layer and at different stages of the ischemic process, demonstrating opposite topographical trends in the stroke nucleus and penumbra. A significant reduction of immunoreactive cells in cortex layers IV–VI on the first and third days of ischemia is typical for all studied neurotrophins. In supragranular layers, their amount remains relatively stable or is slightly reduced as compared with the control. On the eighth day of ischemia, neurotrophins are almost not detected in neurons in the stroke nucleus, while the induction of immunoreactivity occurs in the penumbra. In the penumbra, NT-3-immunoreactive neurons prevail in layers II–III, BDNF is detected in neurons of layers II–III and V, while astrocytes constitute the main population of GDNF-immunoreactive cells. The topography of neurotrophins in the contralateral hemisphere repeats the pattern of their localization in the area of the penumbra. A heterogeneous stratification of neurotrophins and their selective response to ischemic injury are determined by their different involvement in maintaining cytoprotective and neurodestructive effects.

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Funding

This work was carried out within the framework of planned theme no. 01201350008 of the Pacific State Medical University, Ministry of Health of the Russian Federation (Vladivostok).

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Authors and Affiliations

  1. Department of Histology, Embryology, and Cytology, Pacific State Medical University, Ministry of Health of the Russian Federation, 690002, Vladivostok, Russia

    S. G. Kalinichenko, A. V. Korobtsov & N. Yu. Matveeva

Authors
  1. S. G. Kalinichenko
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  2. A. V. Korobtsov
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  3. N. Yu. Matveeva
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Corresponding author

Correspondence to S. G. Kalinichenko.

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Conflict of interest. The authors state that they have no conflict of interest.

Statement on the welfare of animals. The experiments on animals were carried out according to the Directive 2010/63/EU of the European Union in 2010. All the experimental procedures were approved by the interdisciplinary Ethics Committee of Pacific State Medical University, Ministry of Health of the Russian Federation (protocol no. 4 of March 6, 2013).

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Translated by A. Barkhash

Abbreviations: BDNF, brain derived neurotrophic factor; GDNF, glial cell line-derived neurotrophic factor; NT-3, neurotrophin-3; NTs, neurotrophins.

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Kalinichenko, S.G., Korobtsov, A.V. & Matveeva, N.Y. Immunolocalization of BDNF, GDNF, and NT-3 in the Rat Parietal Cortex with Permanent Occlusion of the Middle Cerebral Artery. Cell Tiss. Biol. 16, 213–222 (2022). https://doi.org/10.1134/S1990519X2203004X

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