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Induced Stress on Red Blood Cell Promotes Red Blood Cell-Endothelial Adhesion

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Abstract

Besides disease condition, very few stress stimulants were determined to provoke red blood cell (RBC) adhesion to endothelial cells (EC). However, the possible role of other stress factors which disrupt RBCs anti-adhesive property is still unknown. To resolve this, we studied in vitro static adherence of RBC-EC after RBC exposure to physical (osmotic and shear stress) and chemical stress stimulants (cholesterol depletion and nitric oxide modulation). In support with earlier studies, RBC under hypertonic shock demonstrated a significant increase in RBC-EC adherence as a result of prominent structural modification. Besides, our study shows that shear stress, cholesterol depletion and nitric oxide inhibition in RBC increases RBC-EC adhesiveness which elucidates cholesterol, nitric oxide and shear stress importance in preventing RBC-EC adhesion. Thus, present study shows that assessment of RBC-EC interaction after exerting to external stress is critical in understanding pathophysiological conditions.

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ACKNOWLEDGMENTS

The authors thank Centre for Nanoscience and technology, Anna University, Chennai, Tamil Nadu, India for the instrumental facility.

Funding

This work was supported by a grant from Centre for research, Anna University, India to MV; from the University Grant Commission-Faculty Research Program (UGC-FRP), Government of India to SC; from Department of Science and Technology, Science and Engineering Research Board (SERB), India to NS (Grant no. PDF/2017/000849).

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Authors and Affiliations

  1. Department of Biotechnology, Anna University, Chennai, India

    Mahalakshmi Vijayaraghavan, Prattusha Sengupta & Suvro Chatterjee

  2. AU-KBC Research Centre, Anna University, MIT Campus, Chennai, India

    Mahalakshmi Vijayaraghavan, Prattusha Sengupta, Natarajan Suganya & Suvro Chatterjee

  3. APJ Kalam Center for Excellence, Dr. MGR Educational and Research Institute, Maduravoyal, Chennai, India

    V. N. Sumantran

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  1. Mahalakshmi Vijayaraghavan
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Correspondence to Suvro Chatterjee.

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Statement of compliance with standards of research involving humans as subjects. The study protocol was approved by Institutional Biosafety and Ethical Committee of AU-KBC Research Centre, Chennai, India (Annexure I: Project number III dated July 19, 2016).

AUTHOR’S CONTRIBUTION

MV prepared the manuscript, designed, performed the experiments and analyzed the data; SC supervised, designed the experiments and approved the manuscript. PS helped in performing the experiments. SVN and NS contributed to the discussion and reviewed the manuscript. All authors reviewed the manuscript.

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Abbreviations: DMEM—Dulbecco modified Eagle medium, PBS—phosphate buffer saline, FBS—fetal bovine serum, L‑NAME—N-omega-nitro-L-arginine methyl ester, L-ARG—L-arginine, NaCl—sodium chloride, MβCD- Methylbetacyclodextrin, SFM—serum free medium (DMEM without FBS), SEM—scanning electron microscope.

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Mahalakshmi Vijayaraghavan, Sengupta, P., Sumantran, V.N. et al. Induced Stress on Red Blood Cell Promotes Red Blood Cell-Endothelial Adhesion. Cell Tiss. Biol. 14, 448–457 (2020). https://doi.org/10.1134/S1990519X20060085

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