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Distribution of Some Functional Molecules on T-Lymphocytes in Chronic Lymphoblastic Leukemia

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Abstract—

T-lymphocytes, their subpopulations, and natural killer cells (NCs) were characterized according to the distribution of membrane molecules involved in intercellular interactions and signaling (CD45, CD3, CD4, CD8, CD16, CD56) in patients with chronic lymphocytic leukemia at the stage of disease diagnosis. Current methodological approaches to determine the studied parameters were used, taking into account the characteristics of the lymphoid pool of blood in patients with chronic lymphocytic leukemia. It has been established that the population of T-lymphocytes of the patient peripheral blood is characterized by decreased content of CD45 and CD3 surface receptors. The average level of the membrane CD4 receptor was reduced in lymphocytes belonging to the T-helper subpopulation. The CD8 receptor remained unchanged in subpopulation of cytotoxic T-lymphocytes. CD8 and CD16 molecules were distributed on the surface of NC cells of patients with chronic lymphocytic leukemia at a normal level, while the expression of CD56 was significantly less. The data facilitate understanding of the energy of immune system cells and can be used to predict the course of chronic lymphocytic leukemia.

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Authors and Affiliations

  1. Kirov Research Institute of Hematology and Blood Transfusion of Federal Medical-Biological Agency, 610027, Kirov, Russia

    N. V. Isaeva & E. H. Zotina

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  1. N. V. Isaeva
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  2. E. H. Zotina
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Correspondence to N. V. Isaeva.

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Translated by I. Fridlyanskaya

   Abbreviations: NK—natural killer, ICC—immunocompetent cells, MFI—mean fluorescence intensity, CLL—chronic lymphocytic leukemia, CD—cluster of differentiation, MHC—major histocompatibility complex, TCR—T-cell receptor.

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Isaeva, N.V., Zotina, E.H. Distribution of Some Functional Molecules on T-Lymphocytes in Chronic Lymphoblastic Leukemia. Cell Tiss. Biol. 13, 198–206 (2019). https://doi.org/10.1134/S1990519X19030040

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