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Additional Prognostic Markers of Human Colon Adenocarcinoma

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Abstract—

The expression of potential prognostic markers of human colon adenocarcinoma in tumor and adjacent normal tissue, as well as the relationship between their expression levels was studied. The expression of potential prognostic markers (IGFBP3, AhR, CYP1A1, CYP1B1, HIF-1α, OCT4A, OCT4B, OCT4B1) was assayed with quantitative RT-PCR (qPCR). A correlation between IGFBP3 and AhR expression was found. AhR signaling was analyzed in the primary culture of human colon adenocarcinoma BSK 8 with IGFBP3 gene knockout. It was found that the enhanced expression of AhR can be an additional diagnostic criterion for colon cancer. The correlation between the expression level of HIF-1α, IGFPB3, and OCT4 isoforms and clinic stage (TNM) was not estimated. Clones with IGFBP3 gene knockout had augmented doubling time and increased resistance to the cytostatic drug 5-ftoruracile after modulated activity of dioxin receptor with its agonist—indol-3-carbinol.

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ACKNOWLEDGMENTS

This study was supported by the Russian Science Foundation, project no. 14-50-00068.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    M. A. Bystriakova, S. A. Koshkin & E. N. Tolkunova

  2. St. Petersburg Clinical Scientific and Practical Center of Specialized Medical Care (Oncology-Oriented), 197758, St. Petersburg, Russia

    A. V. Gavrilyukov

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  1. M. A. Bystriakova
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  2. S. A. Koshkin
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Correspondence to E. N. Tolkunova.

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Statement on the welfare of animals. This article does not contain any studies with animals performed by any of the authors.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by I. Fridlyanskaya

Abbreviations: Colorectal cancer—CRC, CSC—cancer stem cells, RT-PCR—reverse transcription polymerase chain reaction.

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Bystriakova, M.A., Koshkin, S.A., Gavrilyukov, A.V. et al. Additional Prognostic Markers of Human Colon Adenocarcinoma. Cell Tiss. Biol. 13, 31–41 (2019). https://doi.org/10.1134/S1990519X19010048

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