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The dipole-modifying effect of styrylpyridinium dyes and flavonoids on model membranes of different lipid compositions

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Abstract

Changes in dipole potential of lipid bilayers ϕd mimicking cell membranes induced by the adsorption of low-molecular-weight amphiphiles, flavonoids (phloretin and quercetin), and styrylpyridinium dyes (RH 421 and RH 237) were measured. A method based on the determination of ionophore-induced transmembrane current was used to evaluate changes in ϕd after modifier addition. The characteristic parameters of the Langmuir adsorption isotherm and the greatest changes in ϕd at an infinitely large concentration of flavonoid and its desorption constant, which reflects the affinity of the flavonoid to the lipid phase, were determined. The slopes of linear dependences of ϕd increasing on the concentration of the styrylpiridinium dyes in membrane-bathing solution were defined. It was found that the dipole-modifying effect of phloretin depends on the charge of the lipids forming the membranes, while the ability of quercetin to reduce ϕd is determined by the initial hydration of the bilayer. The results indicate that there are different mechanisms of the decrease in ϕd upon the adsorption of the tested flavonoids. It was shown that the changes in ϕd at the incorporation of styrylpyridinium dyes into bilayers are determined by the interaction of modifiers with membrane components.

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Abbreviations

DPhPC:

1,2-diphytanoyl-sn-glycero-3-phosphocholine

DPhPS:

1,2-diphytanoyl-sn-glycero-3-phosphoserine

DOPS:

1,2-dioleoyl-sn-glycero-3-phosphoserine

DOPC:

1,2-dioleoyl-sn-glycero-3-phosphocholine

DOPE:

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

SM:

sphingomyelin from pig brain

SPhS:

N-stearoyl phytosphingosine from Saccharomyces cerevisiae

SES:

N-stearoyl-D-erythrosphinganine

CS:

cholesterol

ES:

ergosterol

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Acknowledgements

The study was supported by the Russian Science Foundation (project no. 14-14-00565, sphigolipidcontaining membrane), Program of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology” (phospholipid bilayers). S.S. Efimova was awarded with Russian Presidential Scholarship (SP- 69.2015.4).

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    S. S. Efimova, L. V. Schagina & O. S. Ostroumova

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  1. S. S. Efimova
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  2. L. V. Schagina
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  3. O. S. Ostroumova
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Correspondence to S. S. Efimova.

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Original Russian Text © S.S. Efimova, L.V. Schagina, O.S. Ostroumova, 2017, published in Tsitologiya, 2017, Vol. 59, No. 3, pp. 229–235.

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Efimova, S.S., Schagina, L.V. & Ostroumova, O.S. The dipole-modifying effect of styrylpyridinium dyes and flavonoids on model membranes of different lipid compositions. Cell Tiss. Biol. 11, 335–341 (2017). https://doi.org/10.1134/S1990519X17040058

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  • DOI: https://doi.org/10.1134/S1990519X17040058

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