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Problems with Technologies of Genomic Editing and Transgenesis

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Abstract—This study briefly summarizes the problems that arise when working with transgenesis systems based on recombinases and CRISPR/Cas9. A number of recent results are highlighted regarding the possible effects induced by tamoxifen and doxycycline, agents that are widely used to regulate gene expression in transgenic animals with inducible gene expression. According to these data, there is always the possibility of ectopic tamoxifen-independent expression of the transgene, and currently it is not always possible to predict where it will be observed and how this may affect the final picture of gene expression. A similar problem is present with the use of doxycycline-dependent systems, where the “nonspecific” effects of doxycycline are simply ignored. This work raises the problem of the need to conduct control experiments, taking into account the development of possible side effects associated with both the use of gene expression modulators and the systems that alter the genome, as well as the development of alternative approaches.

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Notes

  1. Frt sites are inserted when Flp recombinase is used.

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ACKNOWLEDGEMENTS

The author expresses his gratitude to M.M. Mikhailova for her critical remarks during preparation of the work and Yu.S. Spivak for her help with designing the figures.

Funding

The work was completed under the state assignment of Ministry of Education and Science of the Russian Federation for 2021–2023.

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, ul. Butlerova 5a, 117485, Moscow, Russia

    A. P. Bolshakov

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  1. A. P. Bolshakov
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Correspondence to A. P. Bolshakov.

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Bolshakov, A.P. Problems with Technologies of Genomic Editing and Transgenesis. Neurochem. J. 15, 333–338 (2021). https://doi.org/10.1134/S1819712421040036

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