Abstract—An increase in the level of ROS during the prenatal period, which is associated with active differentiation of cells, leads to the formation of dose-dependent teratogenic effects. The aim of this study was to determine the relationship between the duration of stress experienced by pregnant female rats in the third trimester with the degree of shift of free radical homeostasis in blood plasma and at different levels of the central nervous system. We analyzed changes in the level of activity of superoxide dismutase, changes in the products of lipid peroxidation and changes in oxidative modification of proteins in homogenates of the cerebral cortex, hypothalamus, cerebellum, and spinal cord of prenatally stressed sexually mature male outbred white rats. To this aim, their dams from 16 to 19 days of pregnancy were exposed daily to immobilization stress in plastic cases for one or three hours. Females of the control groups were kept in standard vivarium conditions with free access to food and water. Prenatal stress influenced the free radical homeostasis of all the CNS regions studied in 90-day-old male rats. More significant effects were observed after 3 hours of immobilization. The offspring of stressed mothers were characterized by more pronounced shifts in free radical balance. As a result of one-hour of daily immobilization from 16 to 19 days of pregnancy, we observed an increase in the indices of oxidative modification of the protein and lipid components in the mature offspring of stressed mothers, while the consequences of three-hour-long prenatal immobilization, on the contrary, are characterized by a decrease in the studied indices of free radical oxidation products and an increase in SOD activity. Regardless of the duration of exposure, the most pronounced shifts in the studied parameters of free radical homeostasis were in the hypothalamic region.
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Kuleshova, O.N., Teply, D.L., Teply, D.D. et al. Dependence of Free Radical Homeostasis of the Central Nervous System of Mature Male Rats on the Duration of Prenatal Stress. Neurochem. J. 15, 159–163 (2021). https://doi.org/10.1134/S1819712421020094
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DOI: https://doi.org/10.1134/S1819712421020094