Abstract—It is known that chaperones and, in particular, 70 kDa heat shock proteins (HSP70), are an integral part of cellular proteostasis. To assess the role of HSP70 in the neurodegenerative process, we used a new hybrid strain of mice with co-expression of the aberrant ΔFUS protein (1–359) and human HSP70. The mice of the new hybrid line are characterized by a longer lifespan, as well as an increase in the duration of the symptomatic stage as compared to control animals. In addition, according to the results of physiological tests, it can be concluded that HSP70 has a positive effect on muscle strength at the symptomatic stage of the model disease.
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The work was supported by the Russian Science Foundation, project no. 19-13-00378 and corresponds to the State Assignment of the Institute of Physiologically Active Substances of the Russian Academy of Sciences 0090-2019-0005 (maintenance and phenotyping of transgenic animals) using the equipment of the Center for Collective Use of the Institute of Physiologically Active Substances of the Russian Academy of Sciences. The mouse strains were created and provided for experiments by the “Genomic Editing” Common Facilities and supported by the Center of high precision editing and genetic technologies for biomedicine of the Institute of Gene Biology of the Russian Academy of Sciences.
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Ethical approval. All manipulations with animals were performed in accordance with the Guide on the maintenance and use of laboratory animals [11]. The protocols of experimental animal research and methods of euthanasia were approved by the Bioethics Commission of Institute of Physiologically Active Substances of the Russian Academy of Sciences (protocol no. 30; April 15, 2019)
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Vikhareva, E.A., Chicheva, M.M., Evgen’ev, M.B. et al. Effect of Heat Shock Proteins on Life Span and Behavioral Functions of Animals with FUS Proteinopathy. Neurochem. J. 15, 37–41 (2021). https://doi.org/10.1134/S1819712421010141
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DOI: https://doi.org/10.1134/S1819712421010141