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Anti-aging Effect of Rutin in Caenorhabditis elegans and D-Gal-Induced Aging Mouse Model

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Caenorhabditis elegans and D-Gal-induced aging mouse model were used to investigate the anti-aging effect of rutin. The effects of different concentrations of rutin (0, 12.5, 25, and 50 μg/mL) on locomotor behavior, reproductive rate, and lifespan of C. elegans were determined. For establishing the aging mouse model, D-Gal (200 mg/kg) was subcutaneously injected into the back of mice, and mice were treated with rutin (200 mg/kg). At the end of treatment, memory and motor function was assessed by nest building test, open field test, and Y-maze. Serum and brain tissue were collected from each mouse to examine the ROS, lipofuscin, MDA, GSH-Px, and SOD levels. The results showed that rutin prolonged the lifespan of C. elegans, and increased the number of eggs of C. elegans (p < 0.05). In addition, rutin significantly improved the exercise capacity in mice (p < 0.05) and significantly reduced brain tissue ROS (p < 0.05) and MDA (p < 0.01) levels. Meanwhile, rutin could enhance the activity of SOD (p < 0.05) and GSH-Px (p < 0.01) significantly in the serum and brain. In summary, rutin exhibits anti-senescence capabilities which could be ascribed to its antioxidant activities.

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Funding

This work was supported by National Natural Science Foundation of China (grant no. 82104188); The Science and Technology Department Fund Project of Guizhou Province (grant no. QIANKEHE-ZK[2023]507); Scientific research project of Education Department of Guizhou Province (grant no. QIANJIAOJI[2022]235); The Science and Technology Department Fund Project of Zunyi City (grant no. ZSKHHZ[2021]284) and Innovation and entrepreneurship training program for college students (grant nos.  S202210661055, S202210661189, and ZYDC202202321).

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Authors and Affiliations

  1. Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, 563099, Zunyi Guizhou, China

    Liang Xinghua, Wang Shuai & Lang Guangping

  2. Special Key Laboratory of Oral Diseases Research, School of Stomatology, Zunyi Medical University, 563099, Zunyi Guizhou, China

    Han Yingying

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  1. Liang Xinghua
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  2. Han Yingying
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Correspondence to Lang Guangping.

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Statement on the welfare of animals. All procedures were approved by the Zunyi Medical University Animal Care and Use Committee.

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Xinghua, L., Yingying, H., Shuai, W. et al. Anti-aging Effect of Rutin in Caenorhabditis elegans and D-Gal-Induced Aging Mouse Model. Dokl Biochem Biophys 513, 350–354 (2023). https://doi.org/10.1134/S1607672923700515

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  • DOI: https://doi.org/10.1134/S1607672923700515

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