Abstract –
The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.
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Abbreviations: ERAD, endoplasmic reticulum-associated protein quality control and degradation system, RTA, ricin toxin subunit A, VTA, viscumin toxin subunit A, real-time PCR, polymerase chain reaction with real-time product detection, RIB, type II ribosome-inactivating protein, ER, endoplasmic reticulum.
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This work was supported by the Russian Science Foundation (project no. 17-14-01338).
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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The proteome was studied using the equipment of the Core Facility “Human Proteome” (Institute of Biomedical Chemistry).
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Translated by M. Batrukova
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Maltseva, D.V., Raigorodskaya, M.P., Tikhonova, O.V. et al. Relationship between the Expression Level of PSMD11 and Other Proteasome Proteins with the Activity of Ricin and Viscumin. Dokl Biochem Biophys 493, 198–200 (2020). https://doi.org/10.1134/S1607672920040080
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DOI: https://doi.org/10.1134/S1607672920040080