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Genotoxicity assessment of low-level doses of gamma radiation with the SOS chromotest and the Ames test

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

This is the first study to present data on the genotoxicity of low γ-irradiation doses for E. coli and S. typhimurium cells obtained using the SOS chromotest and the Ames test. The most pronounced effect was recorded in the first 24 h of γ-irradiation. After 72 h in the Ames test and after 96 h in the SOS chromotest, a significant effect of γ-irradiation on bacterial cells was detected. The absence of genotoxicity at the later stages can be explained by the adaptation of bacterial cells to the conditions of exposure. The findings allow the bacterial test system to be used for studying the effects of low doses at the early stages of exposure to radiation.

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Authors and Affiliations

  1. Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia

    A. Ya. Bolsunovsky & D. V. Dementyev

  2. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 10, Novosibirsk, 630090, Russia

    O. I. Sinitsyna, T. S. Frolova & E. A. Vasyunina

  3. Novosibirsk State University, ul. Pirogova 2, Novosibirsk, 630090, Russia

    O. I. Sinitsyna & T. S. Frolova

  4. Vorozhtsov Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent’eva 9, Novosibirsk, 630090, Russia

    T. S. Frolova

Authors
  1. A. Ya. Bolsunovsky
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  2. O. I. Sinitsyna
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  3. T. S. Frolova
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  4. E. A. Vasyunina
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  5. D. V. Dementyev
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Corresponding author

Correspondence to A. Ya. Bolsunovsky.

Additional information

Original Russian Text © A.Ya. Bolsunovsky, O.I. Sinitsyna, T.S. Frolova, E.A. Vasyunina, D.V. Dementyev, 2016, published in Doklady Akademii Nauk, 2016, Vol. 469, No. 5, pp. 742–745.

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Bolsunovsky, A.Y., Sinitsyna, O.I., Frolova, T.S. et al. Genotoxicity assessment of low-level doses of gamma radiation with the SOS chromotest and the Ames test. Dokl Biochem Biophys 469, 309–312 (2016). https://doi.org/10.1134/S1607672916040190

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  • DOI: https://doi.org/10.1134/S1607672916040190

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