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Impact of tightly focused femtosecond laser pulses on nucleolus-like bodies of mouse GV oocyte and the ability of mouse oocytes to mature

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

Using femtosecond laser radiation, nucleolus-like bodies (NLBs) of mouse oocytes were locally dissected without damage to zona pellucida, cytoplasmic membrane, nuclear membrane, and nucleoplasm surrounding NLB. It was found that, after dissection of 2.7 × 10–11 cm3 of NLB material, which is approximately 5.2% of 10 μm NLB volume, the probability of germinal vesicle oocyte development to metaphase II stage of meiosis decreased 3–7 times compared to the non-treated oocytes. This result indicates that NLB material organization is significant for mouse oocyte maturation.

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Authors and Affiliations

  1. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia

    A. A. Astafev, A. D. Zalesskiy, O. V. Zatsepina, A. N. Kostrov, A. S. Krivoharchenko, A. A. Osychenko, G. A. Serobyan & V. A. Nadtochenko

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    O. V. Zatsepina & V. A. Nadtochenko

  3. Moscow State University, Moscow, Russia

    V. A. Nadtochenko

Authors
  1. A. A. Astafev
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  2. A. D. Zalesskiy
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  3. O. V. Zatsepina
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  4. A. N. Kostrov
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  5. A. S. Krivoharchenko
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  6. A. A. Osychenko
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  7. G. A. Serobyan
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  8. V. A. Nadtochenko
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Corresponding author

Correspondence to V. A. Nadtochenko.

Additional information

Original Russian Text © A.A. Astafev, A.D. Zalesskiy, O.V. Zatsepina, A.N. Kostrov, A.S. Krivoharchenko, A.A. Osychenko, G.A. Serobyan, V.A. Nadtochenko, 2016, published in Doklady Akademii Nauk, 2016, Vol. 467, No. 5, pp. 602–606.

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Astafev, A.A., Zalesskiy, A.D., Zatsepina, O.V. et al. Impact of tightly focused femtosecond laser pulses on nucleolus-like bodies of mouse GV oocyte and the ability of mouse oocytes to mature. Dokl Biochem Biophys 467, 136–140 (2016). https://doi.org/10.1134/S1607672916020162

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  • DOI: https://doi.org/10.1134/S1607672916020162

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