Skip to main content
SpringerLink
Log in

The inhibitors of Arp2/3 complex and WASP proteins modulate the effect of glutoxim on Na+ transport in frog skin

  • Biochemistry, Biophysics and Molecular Biology
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

Using voltage-clamp technique, the involvement of WASP proteins and Arp2/3 complex in the effect of immunomodulator drug glutoxim on Na+ transport in frog skin was investigated. It was shown for the first time that preincubation of the skin with the N-WASP inhibitor wiskostatin or the Arp2/3 complex inhibitor CK-0944666 significantly decreases the stimulatory effect of glutoxim on Na+ transport. The data suggest the involvement of actin filament polymerization and branching in the glutoxim effect on Na+ transport in frog skin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Natochin, Yu.V., Osnovy fiziologii pochki (Fundamentals of Kidney Physiology), Leningrad: Nauka, 1982.

    Google Scholar 

  2. Firsov, D., Robert-Nicoud, M., Gruender, S., et al., J. Biol. Chem., 1999, vol. 274, pp. 2743–2749.

    Article  CAS  PubMed  Google Scholar 

  3. Krutetskaya, Z.I., Lebedev, O.E., Mel’nitskaya, A.V., et al., Dokl. Biol. Sci., 2008, vol. 421, no. 5, pp. 235–238.

    Article  CAS  PubMed  Google Scholar 

  4. Melnitskaya, A.V., Krutetskaya, Z.I., Lebedev, O.E., et al., Cell Tissue Biol, 2010, vol. 4, no. 3, pp. 273–279.

    Article  Google Scholar 

  5. Melnitskaya, A.V., Krutetskaya, Z.I., Lebedev, O.E., et al., Biol. Membr., 2009, vol. 26, no. 4, pp. 320–321.

    CAS  Google Scholar 

  6. Melnitskaya, A.V., Krutetskaya, Z.I., Lebedev, O.E., et al., Cell Tissue Biol., 2012, vol. 6, no. 3, pp. 248–253.

    Article  Google Scholar 

  7. Krutetskaya, Z.I., Mel’nitskaya, A.V., Antonov, V.G., et al., Dokl. Biol. Sci., 2013, vol. 451, pp. 193–195.

    Article  CAS  PubMed  Google Scholar 

  8. Melnitskaya, A.V., Krutetskaya, Z.I., Butov, S.N., et al., Biophysics (Moscow), 2014, vol. 59, no. 5, pp. 718–720.

    Article  CAS  Google Scholar 

  9. Els, W.J. and Chou, K.Y., J. Physiol., 1993, vol. 462, pp. 447–464.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Bouma, G., Burns, S.O., and Thrasher, A.J., Immunobiology, 2009, vol. 214, pp. 778–90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Peterson, J.R., Bickford, L.C., Morgan, D., et al., Nat. Struct. Mol. Biol., 2004, vol. 11, pp. 747–755.

    Article  CAS  PubMed  Google Scholar 

  12. Nolen, B.J., Tomasevic, N., Russell, A., et al., Nature, 2009, vol. 20, pp. 1031–1034.

    Article  Google Scholar 

  13. Karpushev, A.V., Levchenko, V., Ilatovskaya, D.V., et al., Hypertension, 2011, vol. 57, pp. 996–1002.

    Article  CAS  PubMed  Google Scholar 

  14. Ilatovskaya, D.V., Pavlov, T.S., Levchenko, V., et al., FASEB J., 2011, vol. 25, pp. 2688–2699.

    Article  CAS  PubMed  Google Scholar 

  15. Melnitskaya, A.V., Krutetskaya, Z.I., and Lebedev, O.E., Tsitologiya, 2006, vol. 48, no. 10, pp. 817–840.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia

    Z. I. Krutetskaya, A. V. Melnitskaya, V. G. Antonov & A. D. Nozdrachev

Authors
  1. Z. I. Krutetskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Melnitskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. G. Antonov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. D. Nozdrachev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Z. I. Krutetskaya.

Additional information

Original Russian Text © Z.I. Krutetskaya, A.V. Melnitskaya, V.G. Antonov, A.D. Nozdrachev, 2016, published in Doklady Akademii Nauk, 2016, Vol. 467, No. 3, pp. 347–349.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Krutetskaya, Z.I., Melnitskaya, A.V., Antonov, V.G. et al. The inhibitors of Arp2/3 complex and WASP proteins modulate the effect of glutoxim on Na+ transport in frog skin. Dokl Biochem Biophys 467, 102–104 (2016). https://doi.org/10.1134/S1607672916020071

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672916020071

Keywords

Search

Navigation