Abstract
Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with the predominance of particles of 50–150 µm. The surface of these microparticles had a complex topography and ensured efficient cultivation of primary and immortalized fibroblasts. Intradermal injections of microgel suspensions into the area of full-thickness skin wounds did not lead to the development of acute inflammation in mice; instead, they accelerated the recovery of skin tissue and stimulated neurogenesis and angiogenesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Tong, Z., Solanki, A., Hamilos, A., Levy, O., Wen, K., Yin, X., and Karp, J.M., EMBO J., 2015, e201490756.
Animal Cell Culture, Al-Rubeai, M., Ed., Cham Springer Int. Publ., 2015.
Kim, M.H., Kino-oka, M., and Taya, M., Biotechnol. Adv., 2010, vol. 26, no. 1, pp. 7–16.
Ross, A.M., Jiang, Z., Bastmeyer, M., and Lahann, J., Small, 2012, vol. 6, no. 8, pp. 336–355.
Xie, Y., Yang, S.T., and Kniss, D.A., Tissue Eng., 2001, vol. 7, no. 5, pp. 585–598.
Martin, Y., Eldardiri, M., Lawrence-Watt, D.J., and Sharpe, J.R., Tissue Eng. Pt B Rev., 2011, vol. 17, no. 1, pp. 71–80.
Moisenovich, M.M., Pustovalova, O.L., Shackelford, J., Vasiljeva, T.V., Druzhinina, T.V., Kamenchuk, Y.A., Guzeev, V.V., Sokolova, O.S., Bogush, V.G., Deba-bov, V.G., Kirpichnikov, M.P., and Agapov, I.I., Bioma-terials, 2012, vol. 33, no. 15, pp. 3887–3898.
Moisenovich, M.M., Pustovalova, O.L., Arhipova, A.Y., Vasiljeva, T.V., Sokolova, O.S., Bogush, V.G., Deba-bov, V.G., Sevastianov, V.I., Kirpichnikov, M.P., and Agapov, I.I., J. Biomed. Materials Res., 2011, vol. 96, no. 1, pp. 125–131.
Bogush, V.G., Davydova, L.I., Moisenovich, M.M., Sidoruk, K.V., Arkhipova, A.Yu., Kozlov, D.G., Agapov, I.I., Kirpichnikov, M.P., and Debabov, V.G., Appl. Biochem. Microbiol., 2014, vol. 50, no. 8, pp. 780–788.
Moisenovich, M.M., Arkhipova, A.Y., Orlova, A.A., Drutskaya, M.S., Volkova, S.V., Zacharov, S.E., Agapov, I.I., and Kirpichnikov, M.P., Acta Naturae, 2014, vol. 6, no. 1, pp. 96–101.
Bonartsev, A., Yakovlev, S., Boskhomdzhiev, A., Zhar-kova, I., Bagrov, D., Myshkina, V., Mahina, T., Khari-tonova, E., Samsonova, O., Zernov, A., Zhuikov, V., Efremov, Y., Voinova, V., Bonartseva, G., and Shaitan, K., PLoS One, 2013, vol. 8, no. 2, e57200.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © M.M. Moisenovich, N.V. Malyuchenko, A.Y. Arkhipova, M.S. Kotlyarova, L.I. Davydova, A.V. Goncharenko, O.I. Agapova, M.S. Drutskaya, V.G. Bogush, I.I. Agapov, V.G. Debabov, M.P. Kirpichnikov, 2015, published in Doklady Akademii Nauk, 2015, Vol. 463, No. 4, pp. 479–482.
Rights and permissions
About this article
Cite this article
Moisenovich, M.M., Malyuchenko, N.V., Arkhipova, A.Y. et al. Novel 3D-microcarriers from recombinant spidroin for regenerative medicine. Dokl Biochem Biophys 463, 232–235 (2015). https://doi.org/10.1134/S1607672915040109
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672915040109