Abstract
The effect of the chemical structure of crosslinking agents on the activity of proteins in hydrogels obtained by the copolymerization of acrylamide, a crosslinking agent, and an unsaturated protein derivative is studied. It is shown that interaction between units of the hydrophobic crosslinking agent which increases with an increase in the length of the aliphatic region between the double bonds of the crosslinking agent leads to an additional “hydrophobic crosslinking” of macromolecules and causes a decrease in the activity of the immobilized protein.
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REFERENCES
P. Angsantikul and A. Mitragotri, Trends Pharmacol. Sci. 41, 681 (2020).
T. Wang, G. Qu, C. Wang, Y. Cheng, J. Shang, J. Zheng, Z. Feng, Q. Chen, and N. He, Langmuir 35, 13999 (2019).
V. E. Diederich, P. Studer, A. Kern, M. Lattuada, G. Storti, R. I. Sharma, J. G. Snedeker, and M. Morbidelli, Biotechnol. Bioeng. 110, 1508 (2013).
K. Nalampang, R. Panjakha, R. Molloy, and B. J. Tighe, J. Biomater. Sci., Polym. Ed. 24, 1291 (2013).
C. A. Schoener, H. N. Hutson, and N. A. Peppas, J. Biomed. Mater. Res 101, 2229 (2013).
L. I. Valuev, I. L. Valuev, L. V. Vanchugova, and I. L. Obydennova, Polym. Sci., Ser. B 57, 456 (2015).
L. V. Vanchugova, L. I. Valuev, I. L. Valuev, and Yu. A. Talyzenko, Polym/ Sci., Ser. B 55, 77 (2013).
J. Chauvet and R. Acher, J. Biol. Chem. 242, 4274 (1967).
V. V. Mosolov, Proteolytic Enzymes (Nauka, Moscow, 1971) [in Russian].
D. F. Erlanger, N. Kokowski, and W. Cohen, Arch. Biochem. Biophys. 95, 271 (1961).
Y. Cohen, O. Ramon, I. J. Kopelman, and S. J. Mizrah, J. Polym. Sci., Part B: Polym. Phys. 30, 1055 (1992).
H. Rolfes and R. F. T. Stepto, Makromol. Chem.,Theory Simul. 1, 969 (1992).
L. I. Valuev, V. V. Chupov, G. A. Sytov, I. M. Shanazarova, L. N. Kislaya, V. A. Sinani, and N. A. Plate, Vysokomol. Soedin., Ser. A 37, 787 (1995).
N. A. Plate, L. I. Valuev, V. A. Sinani, and V. V. Chupov, Biotekhnologiya 3, 173 (1987).
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This work was carried out within the framework of the state task for the Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences.
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Valuev, I.L., Vanchugova, L.V., Gorshkova, M.Y. et al. Structure of Hydrogels and Activity of Proteins Immobilized in Them. Polym. Sci. Ser. B 63, 404–407 (2021). https://doi.org/10.1134/S1560090421040114
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DOI: https://doi.org/10.1134/S1560090421040114