Abstract
Radiation damage can occur due to the direct effect of radiation on DNA molecules, which accounts for 30–40% of lesions, or by the generation of free radicals that in turn damage DNA, which accounts for 60–70% [1]. A comparative analysis of the induction and repair of DNA single-strand break (SSB) and double-strand break (DSB) in neuronal cells of mice (hippocampus and cerebellum) under γ-irradiation after intraperitoneal administration of 1-β-D-arabino-furanosylcytosine (AraC) and 1-β-D-arabino-furanosylcytosine/hydroxyurea (AraC/HU) combination in vivo was performed. DNA comet assay method was used to study the regularities in the formation of DNA SSB and DSB in cells from hippocampus and cerebellum of mice under the action of γ-radiation in vivo, under the influence of AraC and HU. It was found that for all types of cells used, there is a linear character in the yield of DNA lesions. It has been shown that the amount of DNA SSBs and DSBs formed during irradiation under the influence of AraC significantly increases. An additional increase in the yield of DNA SSBs and DSBs is observed under the combined action of AraC and HU.
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Erhan, SE., Boreyko, A.V., Kozhina, R.A. et al. Induction of DNA Damage in Neuronal Cells of Mice under the Influence of Repair Inhibitors under the Action of Gamma-Rays in vivo. Phys. Part. Nuclei Lett. 19, 586–589 (2022). https://doi.org/10.1134/S1547477122050168
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DOI: https://doi.org/10.1134/S1547477122050168