Abstract
A review of the methods of synthesis of fluorescein is given. An improved method of synthesis of a chromatographically pure fluorescein-5-isothiocyanate nanomarker for labeling immunoglobulins is developed, which will allow creation of high-quality biosensors. A unique method of synthesis of 5-aminofluorescein, a precursor of fluorescein-5-isothiocyanate, was also developed. The properties of fluorescein-5-isothiocyanate were studied to find that it exists in two forms having different solubility.
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Original Russian Text © V.N. Glushko, L.I. Blokhina, N.Yu. Sadovskaya, 2014, published in Rossiiskii Khimicheskii Zhurnal, 2014, Vol. 58, No. 1, pp. 40–45.
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Glushko, V.N., Blokhina, L.I. & Sadovskaya, N.Y. Studies on the synthesis of fluorescein-5-isothiocyanate: A fluorescent nanomarker for biosensors. Russ J Gen Chem 85, 2458–2464 (2015). https://doi.org/10.1134/S1070363215100412
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DOI: https://doi.org/10.1134/S1070363215100412