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E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells

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Abstract

Objective: In recent years, bactofection—bacteria-mediated DNA transfer into mammalian cells is considered a promising approach to cancer gene therapy. Results and Discussion: In this study, we first attempted to develop a probiotic strain Escherichia coli Nissle 1917 for transferring shuttle plasmid into colorectal carcinoma cells HCT116. For this purpose, the strain was modified by inv and hly genes from Yersinia enterocolitica and Listeria monocytogenes. Conclusions: Although the efficiency of HCT116 transfection by GFP-plasmid was exceptionally low, the results demonstrated the potential use the modified probiotic strain for bactofection.

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DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by ongoing institutional funding. No additional grants to carry out or direct thisparticular research were obtained.

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Authors and Affiliations

  1. ITMO University, 191002, St. Petersburg, Russia

    N. Deeb, G. D. Otinov, A. Mohamed, E. R. Gandalipov, E. A. Kovtunov & E. I. Koshel

  2. Penn Sate College of Medicine, Hershey Medical Center, 17033, Hershey, USA

    E. A. Kovtunov

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  1. N. Deeb
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  2. G. D. Otinov
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  3. A. Mohamed
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  4. E. R. Gandalipov
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  5. E. A. Kovtunov
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Correspondence to G. D. Otinov or E. I. Koshel.

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Deeb, N., Otinov, G.D., Mohamed, A. et al. E. Coli Nissle 1917 Based Carrier for DNA Delivery into Colorectal Carcinoma Cells. Russ J Bioorg Chem 50, 459–466 (2024). https://doi.org/10.1134/S1068162024110451

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