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Three-Domain Antibodies against the Tumor Necrosis Factor: Investigation of Their Biological Activity In Vitro

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Abstract—

The aim of this work was to evaluate novel three-domain antibodies consisting of two domains specific for human tumor necrosis factor (hTNF), and of the third domain responsible for binding to myeloid cells. The additional hTNF-binding domain should serve to increase the biological activity of new antibodies. Capacity of these proteins to bind hTNF on the macrophage surface and to neutralize its biological activity in vitro was assessed.

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ACKNOWLEDGMENTS

Part of the sequencing work was performed on the equipment of the Genom CEC of the IMB RAS (http://www.eimb.ru/ru1/ckp/ccu_genome_c.php).

Funding

This work was supported by the state assignment (project 6.6379.2017/8.9), Russian Foundation for Basic Research (project nos. 17-04-01478 and 17-04-01137) and RSF (project no. 19-75-30032).

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Authors and Affiliations

  1. Lobachevsky State University of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    E. A. Vasilenko, E. N. Gorshkova & I. V. Astrakhantseva

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    S. A. Nedospasov

  3. Moscow State University, 119991, Moscow, Russia

    S. A. Nedospasov

  4. Blokhin Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod, 603950, Nizhny Novgorod, Russia

    V. V. Mokhonov

Authors
  1. E. A. Vasilenko
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  2. E. N. Gorshkova
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  3. I. V. Astrakhantseva
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  4. S. A. Nedospasov
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  5. V. V. Mokhonov
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Corresponding author

Correspondence to E. A. Vasilenko.

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COMPLIANCE WITH ETHICAL STANDARDS

In this work, the international principles of care and use of animals were observed.

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The authors declare no conflict of interest.

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Translated by L. Onoprienko

Corresponding author: phone: +7 (831) 462-32-17; e-mail: kat802@rambler.ru.

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Vasilenko, E.A., Gorshkova, E.N., Astrakhantseva, I.V. et al. Three-Domain Antibodies against the Tumor Necrosis Factor: Investigation of Their Biological Activity In Vitro. Russ J Bioorg Chem 46, 299–305 (2020). https://doi.org/10.1134/S1068162020030218

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  • DOI: https://doi.org/10.1134/S1068162020030218

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