Abstract
The antioxidant activity of dihydroquercetin (DHQ) complexes with zinc, copper(II) and calcium was studied in vitro in blood plasma of healthy donors. The state of lipid peroxidation (LPO) in blood plasma was assessed by the content of malondialdehyde (MDA), diene (DC) and triene (TC) conjugates. The effect of DHQ and the complexes on the activity of the catalase enzyme in blood plasma was determined. It was found that DHQ complex with zinc ion reduces the MDA content in blood plasma by 14.9% compared with the control, which is twice as high as for DHQ (7.5%). The corresponding parameters of DHQ complexes with copper(II) and calcium ions were 11.2 and 3.7%, respectively. The effect of the complexes on the decrease in the DC and TC content in blood plasma compared with the control is comparable with the corresponding parameters for DHQ. The DHQ complex with zinc ion increases the catalase activity by 1.5% compared with DHQ. The complexes containing copper(II) and calcium ions increase the catalase activity no more than DHQ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chervyakovskii, E.M., Kurchenko, V.P., and Kostyuk, V.A., The role of flavonoids in biological reactions with electron transfer, Trudy BGU, 2009, vol. 4, no. 1, pp. 9–26.
Babkin, V.A., Ostroukhova, L.A., Babkin, D.V., and Malkov, Yu.A., A method for the obtaining dihydroquercetin, RF Patent no. 2158598, 2000.
Babkin, V.A., Ostroukhova, L.A., and Trofimova, N.N., Biomassa listvennitsy: ot khimicheskogo sostava do innovatsionnykh produktov (Larch Biomass: From Chemical Composition to Innovative Products), Novosibirsk: Izd. SO RAN, 2011.
De Souza, R.F.V. and De Giovani, W.F., Synthesis, spectral and electrochemical properties of Al(III) and Zn(II) complexes with flavonoids, Spectrochim. Acta, Part A, 2005, pp. 1985–1990.
De Souza, R.F. and De Giovani, W.F., Antioxidant properties of complexes of flavonoids with metal ions, Redox Rep., 2004, vol. 9, no. 2, pp. 97–104.
Markovic, J.M.D., Markovic, Z.S., Brdaric, T.P., and Filipovic, N.D., Comparative spectroscopic and mechanistic study of chelation properties of fisetin with iron in aqueous buffered solutions. implications on in vitro antioxidant activity, Dalton Trans., 2011, no. 40, pp. 4560–4571.
Pereira, R.M.S., Andrades, N.E.D., Paulino, N., Sawaya, A.C.H.F., Eberlin, M.N., Marcucci, M.C., Favero, G.M., Novak, E.M., and Bydlowski, S.P., Synthesis and characterization of a metal complex containing naringin and Cu, and its antioxidant, antimicrobial, antiinflammatory and tumor cell cytotoxicity, Molecules, 2007, no. 12, pp. 1352–1366.
Panhwar, Q.K. and Memon, Sh., Synthesis and evaluation of antioxidant and antibacterial properties of morin complexes, J. Coord. Chem., 2011, vol. 64, no. 12, pp. 2117–2129.
Panhwar, Q.K., Memon, Sh., and Bhanger, M.I., Synthesis, characterization, spectroscopic and antioxidation studies of Cu(II)–morin complex, J. Mol. Struct., 2010, vol. 967, pp. 47–53.
Symonowicz, M. and Kolanek, M., Flavonoids and their properties to form chelate complexes, Biotechnol. Food Sci., 2012, vol. 76, no. 1, pp. 35–41.
Tang, H., Wang, X., Yang, S., and Wang, L., Synthesis, characterization and biological activities of Pt(II) and Pd(II) complexes with 2,3,4,5,7-pentahydroxyflavone, Rare Metals, 2004, vol. 23, pp. 38–42.
Panhwar, Q.K. and Memon, Sh., Synthesis and properties of zirconium(IV) and molybdate(II) morin complexes, J. Coord. Chem., 2012, vol. 65, no. 7, pp. 1130–1143.
Panhwar, Q.K. and Memon, Sh., Synthesis, spectral characterization and antioxidant activity of tin(II)–morin complex, Pak. J. Anal. Environ. Chem., 2012, vol. 13, no. 2, pp. 159–168.
Kostyuk, V.A. and Potapovich, A.I., Bioradikaly i bioantioksidanty (Bioradicals and Bioantioxidants), Minsk, 2004.
Trofimova, N.N., Stolpovskaya, E.V., Babkin, V.A., Fedorov, S.V., Kalabin, G.A., Goryainov, S.V., Zolotarev, E.E., Safronov, A.Yu., Kashevskii, A.V., and Zhitov, R.G., The structure and electrochemical properties of metal complexes with dihydroquercetin, Russ. J. Bioorg. Chem., 2015, vol. 41, no. 7, pp. 745–752.
Goncharenko, M.S. and Latinova, A.M., Method for assessing lipid peroxidation, Lab. Delo, 1985, no. 1, pp. 60–61.
Arutyunyan, A.V., Dubinina, E.E., and Zybina, N.N., Metody otsenki svobodnoradikal’nogo okisleniya i antioksidantnoi sistemy organizma. Metodicheskie rekomendatsii (Methods for Assessing Free Radical Oxidation and the Body Antioxidant System: Guidelines), St. Petersburg, 2000.
Korolyuk, M.A., Ivanova, L.I., Maiorova, I.G., and Tokarev, V.E., Method for the determination of catalase activity, Lab. Delo, 1988, no. 1, pp. 16–19.
Trofimova, N.N., Babkin, V.A., and Stolpovskaya, E.V., Method for obtaining mono-and biligand complex compounds of divalent metal ions (zinc, copper(II), and calcium) with dihydroquercetin, with enhanced antioxidant activity, RF Patent no. 2553428, 2015.
Gosudarstvennaya farmakopeya SSSR (State Pharmacopoeia of the USSR), vol. 2: Obshchie metody analiza. Lekarstvennoe rastitel’noe syr’e (General Methods of Analysis. Medicinal Plant Raw Materials), Moscow: Meditsina, 1989.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.V. Stolpovskaya, N.N. Trofimova, V.A. Babkin, 2016, published in Khimiya Rastitel’nogo Syr’ya, 2016, No. 4, pp. 65–70.
Rights and permissions
About this article
Cite this article
Stolpovskaya, E.V., Trofimova, N.N. & Babkin, V.A. Evaluation of Antioxidant Activity of Dihydroquercetin Complexes with Biogenic Metal Ions. Russ J Bioorg Chem 43, 742–746 (2017). https://doi.org/10.1134/S1068162017070160
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1068162017070160