Abstract
We have developed a technology for the production of polymeric nanoparticles containing the incorporated phosphorescent label (europium ions–naphthoyltrifluoroacetone complexes) and streptavidin that is covalently bound on the surface. The aggregation-stable biospecific nanoparticles (40–60 nm in diameter) include up to 2000 molecular tags/particle and retain biological activity and stable phosphorescence for at least 20 months. They can be used in phosphorescence analysis (PHOSPHANTM)-based biochip technology as an effective detector system to record phosphorescence from microzones (microarrays) printed on the well bottoms of standard polystyrene microplates. The creation of a dense monolayer on the surface of a microzone requires up to 108 particles/microarray, or 109 particles/mm2 of area; this is in good agreement with theoretical estimates. The detection limit is as low as 300–400 phosphorescent nanoparticles per a microzone with an area of ~0.1 mm2. It has been demonstrated in the model of thyroid stimulating hormone (TSH) detection in filter paper dried blood that the newly developed detector system is five times more sensitive than the conventional methods of multiplex PHOSPHAN (with Pt-coproporphyrin phosphorescent label) and lanthanide immune fluoroassay (with fluorescent Eu3+ chelate complexes registered in the enhancement solution). The sensitivity of phosphorescent nanoparticle-based detector system is as low as 6.8 × 105 molecules/1.5 μL sample, which corresponds to a TSH concentration of 1.5 × 10–14 M.
Similar content being viewed by others
Abbreviations
- BSA:
-
bovine serum albumin
- DVB:
-
divinylbenzene
- MAA:
-
metacrylic acid
- NTA:
-
2-naphthoyl trifluoroacetone
- PHOSPHAN:
-
phosphorescence analysis
- Pt-CP:
-
Pt-coproporphyrin
- SDS:
-
sodium dodecyl sulfate
- TOPO:
-
trioctylphosphine oxide
- TR-FIA:
-
time-resolved fluoroimmunoassay
- TSH:
-
thyroid-stimulating hormone
References
Osin, N.S., Pomelova, V.G., Sokolov, A.S., Bychenkova, T.A., Bekman, N.I., Sharafudinova, T.Yu., Asliyan, S.K., Ivanovskaya, N.P., Laricheva, S.Yu., and Kanaeva, T.A, Vestnik RAMN, 2007, no. 12, pp. 3–10.
Osin, N.S. and Pomelova, V.G., Frontiers in Research. National Institute of Allergy and Infectious Diseases, NIH, Georgiev, V.St., Western, K.A., and McGowan, J.J., Eds., Totowa, NJ: Humana Press, 2008, pp. 233–240.
Marin, S.J., Merrell, M., Marin, S.J., Merrell, M., and McMillin, G.A., J. Analyt. Toxicol., 2011, vol. 35, no. 1, pp. 40–45.
Troyan, S.L., Kianzad, V., Gibbs-Strauss, S.L., Gioux, S., Matsui, A., Oketokoun, R., Ngo, L., Khamene, A., Azar, F., and Frangioni, J.V, Ann. Surg. Oncol., 2009, vol. 16, no. 10, pp. 2943–2952.
Stafford, P., Halperin, R., Legutki, J.B., Magee, D.M., Galgiani, J., and Johnston, S.A, Mol. Cell Proteomics, 2012, vol. 11, no. 4, p. M111.011593.
Pavkovica, M., Riefkeb, B., Gutberletc, K., Raschkeb, M., and Ellinger-Ziegelbauer, H., J. Pharmacol. Toxicol. Methods, 2014, vol. 69, no. 2, pp. 196–204.
Pomelova, V.G., Korenberg, E.I., Kuznetsova, T.I., Bychenkova, T.A., Bekman, N.I., and Osin, N.S., PLoS ONE, 2015, vol. 10, no. 7, pp. 1–14. doi 10.1371/journal.pone.0130048
Bekman, N.I., Laricheva, S.Yu., Bychenkova, T.A., Pomelova, V.G., and Osin, N.S, Klin. Lab. Diagn., 2015, no. 5, pp. 23–26.
Catalogue of Bioscience (Tianjin) Biotechnology Co., Ltd. www.bioscience-tj.com.
Catalogue of Medisensor, Inc. www.medi-sensor.com.
Catalogue of Lumigenex, Co. LTD. www.lumigenex.com.
Härmä, H., Soukka, T., Lönnberg, S., Paukkunen, J., Tarkkinen, P., and Lövgren, T, Luminescence, 2000, vol. 15, no. 6, pp. 351–355.
Ruckenstein, E, Adv. Polymer Sci., 1997, vol. 127, pp. 1–58.
Yao-Hua van Berkel, K., Entry and the Kinetics of Emulsion Polymerisation, University of Canterbury Christchurch, New Zealand, June 2004.
Reaktsii v polimernykh sistemakh (Reactions in Polymeric Systems), Ivanchev, S.S., Ed., Leningrad: Khimiya, 1987.
Pavlyuchenko, V.N. and Ivanchev, S.S, Usp. Khim., 1981, vol. 50, no. 4, pp. 715–745.
Lee, C.-F, Colloid Polym. Sci., 2002, vol. 280, pp. 116–123.
Tang, J., Ding, T., Daniels, E.S., Dimonie, V.L., Klein, A., and El-Aasser, M.S., J. Appl. Polymer Sci., 2003, vol. 88, pp. 30–41.
Men’shikova, A.Yu., Evseeva, T.G., Sirotkin, A.K., and Shabsel’s, B.M, Zh. Prikl. Khim., 2005, vol. 78, no. 6, pp. 1029–1033.
Graillat, C., Pichotb, C., and Guyov, A, Colloids Surf., 1991, vol. 56, pp. 189–200.
Prokopov, N.I., Gritskova, I.A., Cherkasov, B.P., and Chalykh, A.E, Usp. Khim., 1996, vol. 65, no. 2, pp. 178–192.
Men’shikova, A.Yu., Monodisperse polymeric particles in regulated surface structure, Doctoral (Chem.) Dissertation, St. Petersburg, 2008.
Abramzon, A.A., Bocharov, V.V., and Gaevoi, G.M., Poverkhnostno-aktivnye veshchestva: Spravochnik (Surfactants: A Handbook), Abramzon, A.A. and Gaevii, G.M., Eds., Leningrad: Khimiya, 1979.
Osin, N.S., Pomelova, V.G., Shlyakova, S.Yu., Bulatov, A.A., Osipova, T.A., Sigal, E.R., Koryazova, L.K., and Martynov, A.V, Biotekhnologiya, 1997, nos. 9–10, pp. 49–55.
Sunkara, H.B., Jethmalani, J.M., and Ford, W.T., J. Polymer Sci. Part A: Polymer Chem., 1994, vol. 32, pp. 1431–1435.
Buechler, K.F., Noar, J.B., and Tadesse, L., Fluorescence energy transfer in particles, US Patent no. 6238931 B1, 2001.
Huhtinen, P., Kivela, M., Kuronen, O., Hagren, V., Takalo, H., Tenhu, H., Lovgren, T., and Harma, H, Anal. Chem., 2005, vol. 77, pp. 2643–2648.
Lerner, D., Ricchiero, F., Richard, J., Teychenne, D., and Vaslin, S., Fluorescent latex containing at least two fluorochromes, process for producing it and application thereof, US Patnet no. 5716855, 1998.
Staros, J.V., Wright, R.W., and Swingle, D.M, Anal. Biochem., 1986, vol. 156, no. 1, pp. 220–222.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © D.V. Paramonov, T.S. Kostryukova, T.A. Bychenkova, V.G. Pomelova, N.S. Osin, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 6, pp. 722–731.
Rights and permissions
About this article
Cite this article
Paramonov, D.V., Kostryukova, T.S., Bychenkova, T.A. et al. Biospecific nanoparticles for multiplex phosphorescence analysis (PHOSPHAN). Russ J Bioorg Chem 42, 655–663 (2016). https://doi.org/10.1134/S1068162016060091
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1068162016060091