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Identification of proteins in blood nucleoprotein complexes

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Abstract

Nucleoprotein complexes were isolated from blood plasma by affine chromatography using immobilized polyclonal antihistone antibodies. In blood of healthy subjects the prevalence of histone-containing nucleoprotein complexes, including 170–180 bp DNA, were found, whereas in blood of breast cancer patients the 170–180 bp DNA and the DNA containing more than 6000 bp were present in equal quantities. Using MALDI-TOF mass spectrometry, the proteins from histone-containing nucleoprotein complexes were identified that provided transport of extracellular nucleic acids into blood stream. It was found that nucleoprotein complexes from blood of breast cancer patients contained tumor-associated proteins, such as LDOC1L, ADP/ATP translocase 3, and Lamellipodin. These data confirm generation of part of circulating extracellular DNA by tumor cells.

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Abbreviations

exDNA:

extracellular DNA

DTT:

dithiothreitol

NPC:

nucleoprotein complexes

HSA:

human serum albumin

PBS:

10 mM phosphate buffer and 0.15 M NaCl, pH 7.5

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, pr. Lavrentieva 8, Novosibirsk, 630090, Russia

    S. N. Tamkovich, D. S. Serdukov, O. S. Tutanov & P. P. Laktionov

  2. Novosibirsk National Research State University, pr. Pirogova 2, Novosibirsk, 630090, Russia

    S. N. Tamkovich

  3. International Tomographic Center of the Russian Academy of Sciences, ul. Institutskaya 3, Novosibirsk, 630090, Russia

    T. G. Duzhak

  4. Meshalkin Scientific Research Institute of Circulation Pathology, Ministry of Public Health of the Russian Federation, ul. Rechkunovskaya 15, Novosibirsk, 630090, Russia

    P. P. Laktionov

Authors
  1. S. N. Tamkovich
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  2. D. S. Serdukov
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  3. O. S. Tutanov
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  4. T. G. Duzhak
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  5. P. P. Laktionov
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Corresponding author

Correspondence to S. N. Tamkovich.

Additional information

Original Russian Text © S.N. Tamkovich, D.S. Serdukov, O.S. Tutanov, T.G. Duzhak, P.P. Laktionov, 2015, published in Bioorganicheskaya Khimiya, 2015, Vol. 41, No. 6, pp. 686–695.

The article has been adapted from the report presented at the VIIth All-Russian symposium “Proteins and Peptides”, Novosibirsk, 12–17 July, 2015.

R, correlation coefficient and P, reliability of the analysis.

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Tamkovich, S.N., Serdukov, D.S., Tutanov, O.S. et al. Identification of proteins in blood nucleoprotein complexes. Russ J Bioorg Chem 41, 617–625 (2015). https://doi.org/10.1134/S1068162015060163

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  • DOI: https://doi.org/10.1134/S1068162015060163

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