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Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana

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Abstract

In test systems for diagnostics of type I allergy, recombinant allergens in native conformation are used, because immunoglobulins E (IgE), responsible for the development of this type of allergy, recognize conformational epitopes of protein allergens. To obtain recombinant allergens of the Dermatophagoides farinae house dust mites (HDM), Der f 1 and Der f 2, two systems of heterological expression were used: Escherichia coli and Nicotiana benthamiana plants. IgE from sera of children allergic to HDM were shown to recognize the recombinant Der f 2 protein synthesized in both E. coli and N. benthamiana plants, as well as the recombinant Der f 1 protein produced in N. benthamiana plants, while mature form of Der f 1 produced in E. coli did not interact with IgE.

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Abbreviations

HDM:

house dust mites

SIT:

specific immunotherapy

IgE:

immunoglobulin class E

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    D. Yu. Ryazantsev, P. E. Drobyazina, S. K. Zavriev & E. V. Svirshchevkaya

  2. All-Russian Research Institute for Agricultural Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, Moscow, 127550, Russia

    P. E. Drobyazina

  3. Mechnikov Research Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Malyi Kazennyi per. 5a, Moscow, 105064, Russia

    S. V. Khlgatyan

Authors
  1. D. Yu. Ryazantsev
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  2. P. E. Drobyazina
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  3. S. V. Khlgatyan
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  4. S. K. Zavriev
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  5. E. V. Svirshchevkaya
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Correspondence to D. Yu. Ryazantsev.

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Original Russian Text © D.Yu. Ryazantsev, P.E. Drobyazina, S.V. Khlgatyan, S.K. Zavriev, E.V. Svirshchevkaya, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 4, pp. 468–478.

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Ryazantsev, D.Y., Drobyazina, P.E., Khlgatyan, S.V. et al. Expression of house dust mite allergens Der f 1 and Der f 2 in leaves of Nicotiana benthamiana . Russ J Bioorg Chem 40, 433–442 (2014). https://doi.org/10.1134/S1068162014040116

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  • DOI: https://doi.org/10.1134/S1068162014040116

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