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Evaluation of Chitinolytic and Antibiotic Activity of Streptomyces avidinii Ina 01467 and Micromonospora aurantiaca INA 01468

  • ECOLOGY OF SOIL MICROORGANISMS
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Abstract

The evaluation of antibiotic and chitinolytic activity in actinomycetes isolated from soddy-podzolic soil (Albic Retisol (Loamic)) was carried out in Moscow region (56°07′15″ N, 37°30′54″ E). Isolated strains were classified as Streptomyces avidinii INA 01467 and Micromonospora aurantiaca INA 01468 on the basis of cultural and morphological features and analysis of the 16S rRNA gene. Evaluation of the antibiotic activity of isolated actinomycete cultures showed that the strains exhibit both antibacterial activity against gram-positive bacteria and antifungal activity against the collection strains of fungi Sac. cerevisiae INA 01042, C. albicans ATCC 14053, and F. oxysporum VKPM F-148. The ability to synthesize antifungal compounds increased when growing on a medium with chitin by the Streptomyces avidinii INA 01467 strain, including the phytopathogenic Fusarium oxysporum VKPM F-148 strain. Using the Real-Time PCR (Real-Time-PCR) method, the presence of functional genes shitA, responsible for the synthesis of group A chitinase enzymes, was found in the studied bacterial strains of Streptomyces and Micromonospora. The largest amount of the gene was found in the strain Streptomyces avidinii INA 01467, when growing on chitin, and reaches about 15 × 103 copies per mL. The results demonstrated the presence of chitinase and antibiotic activity in the studied strains, including the activity against phytopathogenic fungi, and this allows using these strains in plant protection technologies and soil bioremediation.

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REFERENCES

  1. V. P. Varlamov, A. V. Il’ina, B. Ts. Shagdarova, A. P. Lunkov, and I. S. Mysyakina, “Chitin/chitosan and its derivatives: fundamental problems and practical approaches,” Biochemistry (Moscow) 85 (Suppl. 1), S154–S176 (2020). https://doi.org/10.1134/S0006297920140084

    Article  Google Scholar 

  2. P. A. Kozhevin, Microbial Populations in Nature (Mosk. Univ., Moscow, 1989) [in Russian].

    Google Scholar 

  3. N. A. Manucharova, A. N. Vlasenko, E. V. Men’ko, and D. G. Zvyagintsev, “Specificity of the chitinolytic microbial complex of soils incubated at different temperatures,” Microbiology (Moscow) 80 (2), 205–215 (2011). https://doi.org/10.1134/S002626171102010X

    Article  Google Scholar 

  4. L. A. Pozdnyakov, A. L. Stepanov, M. E. Gasanov, M. V. Semenov, O. S. Yakimenko, I. K. Suada, I. N. Rai, and N. M. Shchegolkova, “Effect of lignohumate on soil biological activity on the Bali Island, Indonesia,” Eurasian Soil Sci. 53 (5), 653–660 (2020). https://doi.org/10.1134/S1064229320050117

    Article  Google Scholar 

  5. M. M. Aly, A. N. Sediq, A. M. Baghdadi, and R. H. Amasha, “Chitin and chitinases, production, characterization and applications,” IOSR J. Pharm. Biol. Sci. 14, 36043 (2019). https://doi.org/10.9790/3008-1402013643

    Article  Google Scholar 

  6. Y. Bai, V. G. Eijsink, A. M. Kielak, J. A. van Veen, and W. de Boer, “Genomic comparison of chitinolytic enzyme systems from terrestrial and aquatic bacteria,” Environ. Microbiol. 18, 38–49 (2016). https://doi.org/10.1111/1462-2920.12545

    Article  Google Scholar 

  7. Bergey’s Manual of Determinative Bacteriology, Ed. by J. A. Holt et al., Vol. 5: The Actinobacteria (Williams and Wilkins, Baltimore etc, 2012). https://doi.org/10.1002/9781118960608

  8. D. Bhattacharya, A. Nagpure, and R. K. Gupta, “Bacterial chitinases: properties and potential,” Crit. Rev. Biotechnol. 27, 21028 (2007). https://doi.org/10.1080/07388550601168223

    Article  Google Scholar 

  9. M. S. Brzezinska, U. Jankiewicz, A. Burkowska, and M. Walczak, “Chitinolytic microorganisms and their possible application in environmental protection,” Curr. Microbiol. 68, 71–81 (2014). https://doi.org/10.1007/s00284-013-0440-4

    Article  Google Scholar 

  10. N. Dahiya, R. Tewari, and G. S. Hoondal, “Biotechnological aspects of chitinolytic enzymes: a review,” Appl. Microbiol. Biotechnol. 71, 7730782 (2006). https://doi.org/10.1007/s00253-005-0183-7

    Article  Google Scholar 

  11. A. V. Golovchenko, T. A. Gracheva, V. A. Lypcan, T. G. Dobrovol’skaya, and N. A. Manucharova, “Actinomycete complexes in eutrophic peatlands,” Eurasian Soil Sci. 55 (8), 1064–1073 (2022). https://doi.org/10.1134/S1064229322080026

    Article  Google Scholar 

  12. S. Gschwendtner, M. Reichmann, M. Müller, V. Radl, J. C. Munch, and M. Schloter, “Abundance of bacterial genes encoding for proteases and chitinases in the rhizosphere of three different potato cultivars,” Biol. Fertil. Soils 46, 6490652 (2010). https://doi.org/10.1007/s00374-010-0460-1

    Article  Google Scholar 

  13. Y. Han, B. Yang, F. Zhang, X. Miao, and Z. Li, “Characterization of antifungal chitinase from marine Streptomyces sp. DA11 associated with South China sea sponge Craniella australiensis,” Mar. Biotechnol. 11, 132–140 (2009). https://doi.org/10.1007/s10126-008-9126-5

    Article  Google Scholar 

  14. C. F. V. Hobel, G. O. Hreggvidsson, V. T. Marteinsson, F. Bahrani Mougeot, J. M. Einarsson, and J. K. Kristjansson, “Cloning, expression, and characterization of a highly thermostable family 18 chitinase from Rhodothermus marinus,” Extremophiles 9 (1), 53064 (2005). https://doi.org/10.1007/s00792-004-0422-3

    Article  Google Scholar 

  15. D. R. Kashyap, P. K. Vohra, S. Chopra, and R. Tewari, “Application of pectinases in the commercial sector: a review,” Bioresour. Technol. 77, 2150237 (2001). https://doi.org/10.1016/S0960-8524(00)00118-8

    Article  Google Scholar 

  16. M. Kumar, A. Amandeep Brar, M. Yadav, A. Chawade, V. Vivekanand, and N. Nidhi Pareek, “Chitinases-potential candidates for enhanced plant resistance towards fungal pathogens,” Agriculture 8 (88), 2018. https://doi.org/10.3390/agriculture8070088

  17. M. E. Lacombe-Harvey, R. Brzezinski, and C. Beaulieu, “Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution,” Appl. Microbiol. Biotechnol. 102, 7219–7230 (2018). https://doi.org/10.1007/s00253-018-9149-4

    Article  Google Scholar 

  18. N. A. Manucharova, “The microbial destruction of chitin, pectin, and cellulose in soils,” Eurasian Soil Sci. 42 (13), 1526–1532 (2009). https://doi.org/10.1134/S1064229309130146

    Article  Google Scholar 

  19. N. N. Nawani, B. D. Kapadnis, A. D. Das, A. S. Rao, and S. K. Mahajan, “Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2,” J. Appl. Microbiol. 93, 965–975 (2002). https://doi.org/10.1046/j.1365-2672.2002.01766.x

    Article  Google Scholar 

  20. Q. Wang, B. Duan, R. Yang, Y. Zhao, and L. Zhang, “Screening and identification of chitinolytic actinomycetes and study on the inhibitory activity against Turfgrass Root rot disease fungi,” J. Biosci. Med. 3, 56065 (2015). https://doi.org/10.4236/jbm.2015.33009

    Article  Google Scholar 

  21. N. Williamson, P. Brian, and E. M. Wellington, “Molecular detection of bacterial and streptomycete chitinase in the environment,” Antonie Van Leeuwenhoek 78, 315–321 (2000). https://doi.org/10.1023/A:1010225909148

    Article  Google Scholar 

  22. X. Xiao, X. Yin, J. Lin, L. Sun, Z. You, P. Wang, and F. Wang, “Chitinase genes in lake sediments of Ardley Island, Antarctica,” Appl. Environ. Microbiol. 71, 790407909 (2005). https://doi.org/10.1128/AEM.71.12.7904-7909.2005

    Article  Google Scholar 

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Funding

The work was carried out within the framework on state assignment of the Faculty of Soil Science, Lomonosov Moscow State University (registration number 122090800042-2) according the Government of Russian Federation Executive Order no. 2515-r on the date of September 2, 2022 with the aim of implementing the most important innovation project of state significance directed at the formation of integrated national system for monitoring the climatically active substances.

Soil sampling was carried out within the framework on state assignment Soil Microbiomes: Genomic Diversity, Functional Activity, Geography, and Biotechnological Potential, project no. 121040800174-6.

Microbiological study was performed with the financial support of Russian Science Foundation (project no. 21-14-00076).

Determination of antibiotic activity was carried out with the financial support of Ministry of Science and Higher Education of the Russian Federation within the framework of Federal Scientific and technical program for Genetic Technologies Development for 2019–2027 (agreement no. 075-15-2021-1345, unique identifier of the project RF193021X0012).

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Authors and Affiliations

  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    G. A. Teregulova, G. V. Uvarov, M. A. Kovalenko & N. A. Manucharova

  2. Gause Institute of New Antibiotics, 119021, Moscow, Russia

    O. N. Sineva, N. N. Markelova & V. S. Sadikova

Authors
  1. G. A. Teregulova
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  2. O. N. Sineva
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  3. N. N. Markelova
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  4. V. S. Sadikova
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  5. G. V. Uvarov
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  6. M. A. Kovalenko
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  7. N. A. Manucharova
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Correspondence to N. A. Manucharova.

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Translated by T. Chicheva

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Teregulova, G.A., Sineva, O.N., Markelova, N.N. et al. Evaluation of Chitinolytic and Antibiotic Activity of Streptomyces avidinii Ina 01467 and Micromonospora aurantiaca INA 01468. Eurasian Soil Sc. 56, 611–618 (2023). https://doi.org/10.1134/S1064229323600094

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  • DOI: https://doi.org/10.1134/S1064229323600094

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