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Abundance and Taxonomic Diversity of Prokaryotes in Fluvisol and Associated Substrates (Vietnam, Pu Hoat Reserve)

  • SOIL BIOLOGY
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Abstract

The quantitative and qualitative characteristics are given of the soil prokaryotic communities in the Dystric Fluvisol, litter, and “suspended soil” in epiphyte baskets. The number of bacteria (direct luminescence method) varied from 1.1 to 2.6 billion cells/g soil and was the highest in the suspended soil sample and lower in the litter and A and AB horizons of Dystric Fluvisol (brown meadow alluvial soil). Proteobacteria, Actinobacteria, and Acidobacteria predominated in the prokaryotic community (at the phylum level); Chloroflexi, Firmicutes, and Verrucomicrobia were found in smaller amounts; the content of Nitrospirae, Planctomycetes, and Gemmatimonadetes was even lower. Representatives of the Archaea domain were found in the A horizon and in the suspended soil. Their content was significantly lower than that of the Bacteria domain and did not exceed 1%. Archaea were represented by the Thaumarchaeota and Euryarchaeota phyla in the A horizon and by Thaumarchaeota and Woesearchaeota phyla in the suspended soil. Based on the calculated ecological indices (alpha and beta diversity, measures of similarity by Bray-Curtis metrics and weighted UniFrac), it was shown that the microbiome of the suspended soil was closer to the microbiome of A horizon of Dystric Fluvisol than to the microbiome of litter. The metabolically active part of the prokaryotic community represented by the Proteobacteria, Actinobacteria, and Acidobacteria phyla reached its maximum in the suspended soil and was lower in the litter and A horizon of Dystric Fluvisol, and this correlated with the high abundance of these phyla and the significant taxonomic diversity of bacteria in this locus. Functional genes (nifH and alkB) were detected in all studied substrates. The number of copies of functional genes was the highest in the suspended soil sample, which makes this locus promising for isolating the strains with high biotechnological potential.

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ACKNOWLEDGMENTS

We are grateful to administration and stuff of Tropical Centre arranged complex work on the study of biological diversity and ecology of Vietnam forests. Special contribution was made by co-directors of the Main Branch Dang Hong Trien and A.N. Kuznetsov and codirectors of Southern Branch Nguyen Van Thien and I.V. Pal’ko. Invaluable help was given by S.P. Kuznetsova and Pham Thi Ha Giang.

Particular thanks we want to express to the administrations and stuff of forest stations of specially protected territories of Vietnam, which provide the opportunities for research, for their invaluable help and comfort conditions.

Funding

This study was carried out within the framework of state assignment of the Ministry of Science and Higher Education of the Russian Federation (projects nos. 121040800174-6 and 121032300081-7) and partly supported by the program of the Interdisciplinary Scientific and Educational School of Moscow University “Future of the Planet and Global Environmental Changes.” Analysis of microbiomes was carried out with support of the Program of Scientific and Technological Development of the Russian Federation “Analysis of Microbiomes of Plants and Invertebrates of Extreme Habitats in Order to Develop Strains Producing New Metabolites and Enzymes” (project no. 075-15-2021-1396). The real-time PCR analysis was supported by the Russian Science Foundation, project no. 21-14-00076.

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Authors and Affiliations

  1. Lomonosov Moscow State University, 119991, Moscow, Russia

    A. V. Kniazeva, L. V. Lysak, N. A. Manucharova, E. V. Lapygina & A. V. Aleksandrova

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, 142290, Pushchino, Russia

    A. V. Kniazeva

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  1. A. V. Kniazeva
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Correspondence to A. V. Kniazeva or L. V. Lysak.

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Translated by T. Chicheva

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Kniazeva, A.V., Lysak, L.V., Manucharova, N.A. et al. Abundance and Taxonomic Diversity of Prokaryotes in Fluvisol and Associated Substrates (Vietnam, Pu Hoat Reserve). Eurasian Soil Sc. 55, 1460–1469 (2022). https://doi.org/10.1134/S1064229322100076

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