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Development of Microbial Consortium for Bioremediation of Oil-Contaminated Soils in the Middle Ob Region

  • REMEDIATION OF POLLUTED SOILS
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Abstract

Hydrocarbon-decomposing microorganisms identified as representatives of the genera Pseudomonas, Rhodococcus, Acinetobacter, Kocuria, Raoultella, and Candida have been isolated from the oil-contaminated soil samples of the Middle Ob region. They have been screened for the ability to decompose various classes of hydrocarbons in a wide temperature range (6–37°C), in acid media (up to pH 4), and at increased salinity (up to 3%), for the ability to produce biosurfactants, and for the presence of genes encoding enzymes responsible for hydrocarbon decomposition. A microbial consortium has been suggested as the basis of a biological preparation for bioremediation of oil-contaminated soils in the Middle Ob region, including strains of Candida fluviatilis 24p-51, Rhodococcus erythropolis 24-44, Acinetobacter calcoaceticus 7-43, and Pseudomonas extremaustralis 7-31. The modes of cultivation and lyophilization of biomass have been determined for these microorganisms. The efficiency of degradation of oil hydrocarbons by the developed microbial consortium has been evaluated in laboratory model systems. The degree of oil degradation by the microbial consortium in the liquid mineral medium was 56%; in the model soil, 22% in 10 days at 24°C.

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Funding

This work was carried out within the framework of state assignment of the Ministry of Science and Higher Education of the Russian Federation no. № 121041300098-7 and, partly, within the framework of the development program of the Interdisciplinary Scientific and Educational School of Lomonosov Moscow State University “Future of the Planet and Global Environmental Changes.”

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Federal Research Center, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    A. A. Vetrova, I. F. Puntus, O. I. Sazonova, S. L. Sokolov, K. V. Petrikov, Ya. A. Delegan, V. A. Samoylenko & A. E. Filonov

  2. Lomonosov Moscow State University, 119991, Moscow, Russia

    S. Ya. Trofimov, R. R. Kinzhaev, N. A. Avetov, A. V. Arzamazova & R. A. Streletskii

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  1. A. A. Vetrova
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  2. S. Ya. Trofimov
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  3. R. R. Kinzhaev
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  4. N. A. Avetov
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  7. O. I. Sazonova
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  8. S. L. Sokolov
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Correspondence to A. A. Vetrova.

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Vetrova, A.A., Trofimov, S.Y., Kinzhaev, R.R. et al. Development of Microbial Consortium for Bioremediation of Oil-Contaminated Soils in the Middle Ob Region. Eurasian Soil Sc. 55, 651–662 (2022). https://doi.org/10.1134/S1064229322050106

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