Abstract
Three varieties (Aura, Magie, and Indra) of soybean (Glycine max [L.] Merr.) were grown in a small-plot experiment on a calcic chernozem with the application of two types of nitrogen fertilizers: ammonium nitrate (Nan) or carbamide (Nc). These fertilizers at the rate of 20 kg N/ha were applied before sowing together with potassium phosphate (60 kg P2O5/ha). The microbial nitrification capacity and the activity of enzymes related to the nitrogen cycle (urease and nitrate reductase) were measured in the rhizosphere (0–20 cm) at the stage of soybean flowering. It was determined that the biological (enzyme) activity of the calcic chernozem in the soybean rhizosphere was more intense on the plots with the Nan fertilizer than on the plots with the Nc fertilizer. The urease activity depended on the type of nitrogen fertilizer (Nan or Nc) under the conditions of soil water deficiency. In the soil under the Aura variety, the urease activity was significantly lower in the treatments with Nc application, and this was accompanied by a decrease in the crop yield. The nitrification capacity of the calcic chernozem was generally low; in the case of the Nc fertilizer, it was significantly lower than in the case of the Nan fertilizer. The nitrate reductase activity of the soil was also lower in the case of the Nc fertilizer. Each of the three soybean varieties had its own response to changes in the nitrogen nutrition aimed at improving the soybean tolerance to fluctuations in the soil water content during the growing season.
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References
E. V. Arinushkina, Manual on the Chemical Analyses of Soils (Moscow State University, Moscow, 1970) [in Russian].
I. V. Boagii, “Influence of inoculation of seeds, microelements, and foliar nutrition on soya productivity,” in Rolul Culturilor Leguminoase i Furajere în Agricultura Republicii Moldova, Ed. by V. Vozian et al. (Tipogr. Central, Chisinau, 2010), pp. 217–222.
N. S. Balaur, A. M. Balter, V. A. Vorontsov, and S. I. Toma, Mechanism of Intake and Transport of Mineral Elements: Energy Aspects (Chisinau, 1991), pp. 79–80.
R. E. Eleshev and Z. B. Bakenova, “Changes in the biological activity of chestnut soils upon the long-term application of fertilizers in a rotation with oil-bearing crops,” Eurasian Soil Sci. 45(11), 1081–1085 (2012).
E. E. Emnova, O. V. Daraban, I. V. Bizgan, and S. I. Toma, “Effect of phosphoric fertilizer and starter rates of nitrogen fertilizers on the phosphatase activity in the rhizosphere soil and nonlignified soybean roots under drought conditions,” Eurasian Soil Sci. 47(2), 102–109 (2014). doi: 10.7868/S0032180X14020063
E. E. Emnova, S. I. Toma, I. V. Bizgan, and O. V. Daraban, “Urease activity in rhizosphere of soya plants at the drought,” Agrokhimiya, No. 3, 75–82 (2014).
E. E. Emnova, S. I. Toma, O. V. Daraban, I. V. Bizgan, and A. B. Budak, “Influence of mineral fertilizers in hydrolytic activity in soya roots at different moistening regimes of carbonate chernozems,” Agrokhimiya, No. 11, 78–86 (2013).
A. I. Ermakov and V. V. Arasimovich, Biochemical Analysis of the Plants (Agropromizdat, Leningrad, 1987) [in Russian].
S. L. Iodko and I. N. Sharkov, “New modification of determination of nitrates in soil using disulfophenolic acid,” Agrokhimiya, No. 4, 95–97 (1994).
V. N. Kudeyarov and V. M. Semenov, “Assessment of the current contribution of fertilizers to the agrogeochemical cycles of nitrogen, phosphorus, and potassium,” Eurasian Soil Sci. 37(12), 1279–1285 (2004).
Laboratory Manual on Agrochemistry, Ed. by V. G. Mineev (Moscow State University, Moscow, 1989) [in Russian].
A. S. Radov, I. V. Pustovoit, and A. V. Korol’kov, Manual on Agrochemistry (Agropromizdat, Moscow, 1978) [in Russian].
S. I. Toma, S. G. Veliksar, T. G. Kudrev, and T. I. Kovacheva, “Influence of boron and molybdenum on sunflower plants at different nitric nutrition,” Izv. Akad. Nauk Mold. SSR, Biol. Khim. Nauki, No. 6, 16–22 (1986).
S. I. Toma, B. F. Palariya, S. S. Lisnik, I. G. Karabadzhak, “Influence of molybdenum, gibberellin, and kartolin on soya productivity at irrigation,” in Physiological and Biochemical Role of Microelements in Control of Adaptive Reactions of the Plants (Shtiintsa, Chisinau, 1990), pp. 170–175.
A. P. Ustyuzhanin, “Strategy of development of the soya production capacities in Russia,” Zemledelie, No. 3, 3–6 (2010).
F. Kh. Khaziev, Manual of Soil Enzymology (Nauka, Moscow, 1990) [in Russian].
F. Kh. Khaziev, System Ecological Analysis of Enzymatic Activity of Soils (Nauka, Moscow, 1982) [in Russian].
T. E. Khomutova, T. S. Demkina, N. N. Kashirskaya, and V. A. Demkin, “Phosphatase activity in the surface and buried chestnut soils of the Volga-Don interfluve,” Eurasian Soil Sci. 45(4), 423–428 (2012).
V. M. Chikanova, Bacterial Fertilizers (Uradzhai, Minsk, 1988) [in Russian].
M. Ya. Shkol’nik, A Role of Microelements in the Plants (Nauka, Leningrad, 1974) [in Russian].
M. Ashraf, N. A. Akram, F. Al-Qurainy, and M. R. Foolad, “Drought tolerance: roles of organic osmolytes, growth regulators, and mineral nutrients,” Adv. Agron. 111(5), 249–296 (2011). doi 10.1016/B978-0-12-387689-8.00002-3
E. Emnova, S. Toma, O. Daraban, and Ia. Druta, “Enzyme activity in soybean root-adhering soil in dependence on nutrition and water content condition,” in Lucrari Stiintifice. Seria Agronomie (Ion Ionescu de la Brad, Iasi, 2012), Vol. 55(2), pp. 51–54.
T. Furdui, T. Tudorache, S. Andries, and V. Botnari, “Impactul secetei si desertificarii asupra agriculturii: modalitati i actiuni de atenuare,” Agric. Moldov., No. 7–8, 12–14 (2012).
M. Iacobuta, V. Vozian, N. Pintelei, and D. Glatchi, “Comportarea soiurilor noi de soia in conditiile de stres climatic ale a. 2009,” in Rolul Culturilor Leguminoase si Furajere in Agricultura Republicii Moldova, Ed. by V. Vozian et al. (Tipografia Centrala, Chisinau, 2010), pp. 107–111.
Instructiuni Metodice Privind Cartarea Agrochimic a Solurilor (Pontos, Chisinau, 2007).
J. Jin, G. Wang, X. Liu, X. Pan, and S. J. Herbert, “Phosphorus affects the soybean, root response to water deficit at initial flowering and full pod stages,” Soil Sci. Plant Nutr. 51, 953–960 (2005).
S. X. Li, Z. H. Wang, and B. A. Stewart, “Responses of crop plants to ammonium and nitrate N,” Adv. Agron. 116(5), 205–397 (2013). doi: 10.1016/B978-0-12-405942-9.00005-0
A. Rusu, “Mǎsuri şi posibilitǎţi de majorare a aportului de materie organicǎ în solurile arate ale Republicii Moldova,” in Cernoziomurile Moldovei — Evolu ia Protec ia şi Restabilirea Fertilit ii Lor (Reclama, Chisinau, 2013), pp. 304–308.
Aplicarea Ingrasamintelor in Agricultura Durabila: Indrumar Practic), Ed. by S. Toma (Tipografia A.S.M., Chisinau, 2008).
V. Vozian, M. Iacobuta, M. Taran, and D. Glatchi, “Studiul influen ei secetei asupra productivityǎţii soiei în 2000–2009,” in Rolul Culturilor Leguminoase şi Furajere in Agricultura Republicii Moldova (Tipografia Centrala, Balti, 2010), pp. 362–365.
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Original Russian Text © E.E. Emnova, O.V. Daraban, Ya.V. Bizgan, S.I. Toma, V.I. Vozian, M.D. Iacobuta, 2015, published in Pochvovedenie, 2015, No. 5, pp. 571–579.
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Emnova, E.E., Daraban, O.V., Bizgan, Y.V. et al. Effect of the nitrogen fertilizer type on the enzyme activity in the rhizosphere of calcic chernozem and soybean production. Eurasian Soil Sc. 48, 509–517 (2015). https://doi.org/10.1134/S1064229315030047
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DOI: https://doi.org/10.1134/S1064229315030047