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Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde

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Abstract

We study the effect of the concentration of glutaraldehyde that can cross-link type I collagen molecules on the collagen stability, mechanical strength, and cytotoxicity. It is shown that collagen containing 10 and 18% of glutaraldehyde exhibits the highest stability in physiological conditions. The results of rheological analysis demonstrate that, for the minimal concentration of glutaraldehyde (1%), mechanical characteristics of collagen hydrogels are improved significantly. However, the glutaraldehyde concentration higher than 10% reduces the vitality of cells by 50%.

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Funding

The preparation of collagen samples and biological tests were supported by the Russian Foundation for Basic Research, project no. 20-03-00400_a. Mechanical tests were supported by the Russian Science Foundation, project no. 19-73-30003.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    Yu. A. Nashchekina, O. A. Lukonina, D. M. Darvish & N. A. Mikhailova

  2. Ioffe Institute, 194021, St. Petersburg, Russia

    Yu. A. Nashchekina & A. V. Nashchekin

  3. Peter the Great St. Petersburg Polytechnic University, 195251, St. Petersburg, Russia

    V. Yu. Elokhovskii & V. E. Yudin

Authors
  1. Yu. A. Nashchekina
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  2. O. A. Lukonina
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  3. D. M. Darvish
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  4. A. V. Nashchekin
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  5. V. Yu. Elokhovskii
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  6. V. E. Yudin
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  7. N. A. Mikhailova
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Corresponding author

Correspondence to Yu. A. Nashchekina.

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The authors declare that they have no conflicts of interest.

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Translated by N. Wadhwa

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Nashchekina, Y.A., Lukonina, O.A., Darvish, D.M. et al. Biological and Rheological Properties of Collagen Cross-Linked with Glutaraldehyde. Tech. Phys. 65, 1535–1540 (2020). https://doi.org/10.1134/S1063784220090224

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  • DOI: https://doi.org/10.1134/S1063784220090224

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