Skip to main content
SpringerLink
Log in

Study of the Influence of a Precipitant Cation on the Formation of Oligomers in Crystallization Solutions of Lysozyme Protein

  • DIFFRACTION AND SCATTERING OF IONIZING RADIATIONS
  • Published:
Crystallography Reports Aims and scope Submit manuscript

Abstract

The influence of the precipitant cation type in a series of chlorides (NaCl, KCl, LiCl, NiCl2, CuCl2, CoCl2) on the structure of lysozyme solutions has been investigated by small-angle X-ray scattering (SAXS) under the conditions favorable for tetragonal crystal growth. The influence of the precipitant concentration on the volume fraction of the oligomers (dimers, octamers) formed before the protein crystallization has been studied. Along with monomers, significant amount of dimers (3.5–13.1%) and octamers (1.7–3.6%) is found in the lysozyme solutions when adding a precipitant. The volume fractions of all oligomers increase with an increase in the precipitant concentration and with a decrease in temperature. In the series of monovalent and divalent ions, the octamer volume fraction increases as K+ – Na+ – Li+ and Cu2+ – Ni2+ – Co2+, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

REFERENCES

  1. A. McPherson and J. A. Gavira, Acta Crystallogr. F 70, 2 (2014).

    Article  Google Scholar 

  2. M. V. Kovalchuk, A. E. Blagov, Y. A. Dyakova, et al., Cryst. Growth Des. 16 (4), 1792 (2016).

    Article  Google Scholar 

  3. Yu. A. D’yakova, K. B. Il’ina, P. V. Konarev, et al., Crystallogr. Rep. 62 (3), 364 (2017).

    Article  ADS  Google Scholar 

  4. A. S. Boikova, Yu. A. D’yakova, K. B. Il’ina, et al., Crystallogr. Rep. 62 (6), 837 (2017).

    Article  ADS  Google Scholar 

  5. A. S. Boikova, Yu. A. D’yakova, K. B. Il’ina, et al., Crystallogr. Rep. 63 (6), 865 (2018).

    Article  ADS  Google Scholar 

  6. A. Ducruix, J. P. Guilloteau, and M. Ries-Kautt, J. Cryst. Growth 168 (1–4), 28 (1996).

    Article  ADS  Google Scholar 

  7. P. Pernot, A. Round, R. Barrett, et al., J. Synchrotron Radiat. 20, 660 (2013).

    Article  Google Scholar 

  8. A. Round, F. Felisaz, L. Fodinger, et al., Acta Crystallogr. D 71, 67 (2015).

    Article  Google Scholar 

  9. M. E. Brennich, J. Kieffer, G. Bonamis, et al., J. Appl. Crystallogr. 49, 203 (2016).

    Article  Google Scholar 

  10. P. V. Konarev, V. V. Volkov, A. V. Sokolova, et al., J. Appl. Crystallogr. 36, 1277 (2003).

    Article  Google Scholar 

  11. P. V. Konarev, M. V. Petoukhov, V. V. Volkov, et al., J. Appl. Crystallogr. 39, 277 (2006).

    Article  Google Scholar 

  12. M. V. Petoukhov, D. Franke, A. V. Shkumatov, et al., J. Appl. Crystallogr. 45, 342 (2012).

    Article  Google Scholar 

  13. D. Franke, M. V. Petoukhov, P. V. Konarev, et al., J. Appl. Crystallogr. 50, 1212 (2017).

    Article  Google Scholar 

  14. D. I. Svergun, C. Barberato, and M. H. J. Koch, J. Appl. Crystallogr. 28, 768 (1995).

    Article  Google Scholar 

  15. Yu. V. Kordonskaya, V. I. Timofeev, Yu. A. D’yakova, et al., Crystallogr. Rep. 63 (6), 947 (2018).

    Article  ADS  Google Scholar 

  16. A. M. Hyde, S. L. Zultanski, J. H. Waldman, et al., Org. Process Res. Dev. 21, 1355 (2017).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 119333, Moscow, Russia

    Yu. A. Dyakova, A. S. Boikova, K. B. Ilina, P. V. Konarev, M. A. Marchenkova, Yu. V. Pisarevsky, V. I. Timofeev & M. V. Kovalchuk

  2. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia

    Yu. A. Dyakova, A. S. Boikova, K. B. Ilina, P. V. Konarev, M. A. Marchenkova, Yu. V. Pisarevsky, V. I. Timofeev & M. V. Kovalchuk

  3. St. Petersburg State University, 199034, St. Petersburg, Russia

    M. V. Kovalchuk

Authors
  1. Yu. A. Dyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. S. Boikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. B. Ilina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. V. Konarev
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. A. Marchenkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. V. Pisarevsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. I. Timofeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. V. Kovalchuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. B. Ilina.

Additional information

Translated by Yu. Sin’kov

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dyakova, Y.A., Boikova, A.S., Ilina, K.B. et al. Study of the Influence of a Precipitant Cation on the Formation of Oligomers in Crystallization Solutions of Lysozyme Protein. Crystallogr. Rep. 64, 11–15 (2019). https://doi.org/10.1134/S1063774519010061

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1063774519010061

Search

Navigation