Abstract
The spectrum of proteinases and the dynamics of proteinase activity in the ambulacrum structures were studied by quantitative gel zymography in the holothurians Eupentacta fraudatrix and Cucumaria japonica under normal conditions and after transverse cutting of the ambulacrum. Proteinases with gelatinolytic activity were detected only in E. fraudatrix; the species had two pools of proteinases. The high-molecular-weight pool was represented by only one (132 kDa) or two (132 and more than 250 kDa) proteins; the low-molecular-weight pool included two proteins with molecular weights of 53 and 47 kDa. In their ability to lyse gelatin, but not casein, and in their sensitivity to inhibitors of different classes of enzymes, gelatinases of E. fraudatrix are similar to vertebrate matrix metalloproteinases. It was shown that the activity of proteinases increased after injury to the animal and that GM6001, a selective inhibitor of metalloproteinases, blocks the proteinase activity. It was suggested that proteinases with gelatinolytic activity are a key factor of regeneration in holothurians.
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ACKNOWLEDGMENTS
The authors express their sincere thanks to Dr. I. Yu. Dolmatov (Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences) for his help in performance of the experiments, for participation in discussions of the results, and constant attention to our work.
Funding
This work was supported by the Russian Foundation for Basic Research, grant no. 20-04-00574.
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Shulga, A.P., Lamash, N.E. Proteinases with Gelatinase Activity and their Role in Ambulacrum Regeneration in Holothurians Eupentacta fraudatrix (D’yakonov and Baranova, 1958) and Cucumaria japonica (Semper, 1868) (Echinodermata: Holothuroidea). Russ J Mar Biol 46, 461–471 (2020). https://doi.org/10.1134/S1063074020060097
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DOI: https://doi.org/10.1134/S1063074020060097