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The enzymes of a marine bacterial isolate from the brown alga Sargassum polycystum agardh, 1821, that catalyzes the transformation of polyanionic oligo-and polysaccharides

  • Microbiology
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Abstract

A search for enzymes involved in the degradation of polyanionic polysaccharides (fucoidans and alginic acid) was conducted among bacterial epiphytes of the brown alga Sargassum polycystum that grows in the territorial waters of the Socialist Republic of Vietnam. Two resistant bacterial strains, F10 and F14, have been isolated from the algal microflora that degrade the thallus of the alga under laboratory conditions. These bacterial strains differed in the morphological, physiological, and biochemical characteristics and in the composition of enzymes. The strains were studied for the ability to synthesize intracellular oligo-and polysaccharide hydrolases and alginate lyases. The optimal conditions for the growth of bacterial strain F14 and the biosynthesis of fucoidanase and polymannuronate-specific alginate lyase were determined. The partially purified alginate lyase was stable at a temperature up to 40°C and had an optimal pH 6.0 and an optimal temperature 35°C.

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Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    Yu. V. Dubrovskaya, V. V. Kurilenko, I. Yu. Bakunina, T. N. Zvyagintseva & V. V. Mikhailov

  2. Nhatrang Institute of Technology Research and Application, Vietnamese Academy of Science and Technology, Nhatrang, Vietnam

    Cao Thi Thuy Hang & Bui Minh Ly

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  1. Yu. V. Dubrovskaya
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  2. V. V. Kurilenko
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  3. Cao Thi Thuy Hang
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  4. Bui Minh Ly
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  5. I. Yu. Bakunina
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  6. T. N. Zvyagintseva
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  7. V. V. Mikhailov
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Correspondence to Yu. V. Dubrovskaya.

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Original Russian Text © Yu.V. Dubrovskaya, V.V. Kurilenko, Cao Thi Thuy Hang, Bui Minh Ly, I.Yu. Bakunina, T.N. Zvyagintseva, V.V. Mikhailov, 2017, published in Biologiya Morya.

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Dubrovskaya, Y.V., Kurilenko, V.V., Hang, C.T.T. et al. The enzymes of a marine bacterial isolate from the brown alga Sargassum polycystum agardh, 1821, that catalyzes the transformation of polyanionic oligo-and polysaccharides. Russ J Mar Biol 43, 392–399 (2017). https://doi.org/10.1134/S1063074017050030

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