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The Problem of Terminal Differentiation and Apoptosis during Human Keratinocytes’ Cryostorage in Suspension

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Abstract

Cryostorage of human epidermal keratinocytes is an essential stage in living epidermal equivalent’s manufacturing. However, cryostorage of keratinocytes in suspension stimulates processes of terminal differentiation and apotosis leading to massive loss of stem and progenitor keratinocytes. Suspension-induced signal of disruption between β1 integrin and extracellular matrix causes simultaneous activation of PI3K/Akt- and Caspase-8 pathways. It is assumed that terminal differentiation could be blocked by fibronectin, PI3-kinase, and Caspase-8-inhibitors added to culture medium after cryostorage in suspension.

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Funding

This work was supported by the Russian Science Foundation (project no. 21-74-30015).

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Authors and Affiliations

  1. Koltzov Institute of Development Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    A. N. Popova & E. A. Vorotelyak

  2. Moscow State University, 119234, Moscow, Russia

    E. A. Vorotelyak

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  1. A. N. Popova
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  2. E. A. Vorotelyak
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Correspondence to A. N. Popova or E. A. Vorotelyak.

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This article does not contain a description of the research carried out by the authors with the participation of humans or the use of animals as objects.

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Popova, A.N., Vorotelyak, E.A. The Problem of Terminal Differentiation and Apoptosis during Human Keratinocytes’ Cryostorage in Suspension. Russ J Dev Biol 52, 215–219 (2021). https://doi.org/10.1134/S1062360421040068

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  • DOI: https://doi.org/10.1134/S1062360421040068

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