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The Mechanism of MSX1 and PAX9 Implication in Tooth Development Based on the Weighted Gene Co-Expression Network Analysis

  • MOLECULAR BIOLOGY OF DEVELOPMENT
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Abstract

In this study, we investigated the mechanism by which Msx1 and Pax9 affect tooth development in mice. The microarray data GSE32321, which contains Msx1 and Pax9 wildtype and knockout samples from mice oral epithelium (Epi) and dental mesenchymal (Mes) cells, was used to identify the differentially expressed genes (DEGs). The highest associated gene modules were then explored in the Epi-Msx, Epi-Pax, Mes-Msx, and Mes-Pax groups by weighted gene co-expression network analysis (WGCNA). Gene Ontology (GO) database analysis and hub gene screening were performed on the modules with the highest relevance. A total of 1467, 986, 1212, and 1293 DEGs were identified in the Epi-Msx, Epi-Pax, Mes-Msx, and Mes-Pax groups, respectively. Four highest associated gene modules were identified. GO enrichment analysis showed that the negative regulation of cell proliferation, cell adhesion, blood vessel development, and blood vessel morphogenesis was involved in tooth development. We further identified the hub genes IDH3A, SSPN, F13A1, and CBLN1, and found that gene expression values varied at different time points during tooth development. Moreover, IDH3A and CBLN1 were shown to be involved in oxidoreduction coenzyme metabolic processes and cell-cell adhesion. Overall, Msx1 and Pax9 play an important role in tooth development in mice, an effect which is probably associated with their regulation of IDH3A, SSPN, F13A1, and CBLN1.

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Authors and Affiliations

  1. Department of Stomatology, Dahua Hospital, Xuhui District, 200237, Shanghai, China

    Feng Wang

  2. Department of Oral and Maxillofacial Head and Neck Oncology, Division Radiation Oncology, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai JiaoTong University School of Medicine, 200011, Shanghai, China

    Wen Jiang & Rongrong Li

  3. National Clinical Research Center for Oral Diseases, 200011, Shanghai, China

    Wen Jiang & Rongrong Li

  4. Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, 200011, Shanghai, China

    Wen Jiang & Rongrong Li

  5. Department of Otolaryngology Head and Neck Surgery, Shanghai Ninth People’s Hospital, Affiliated to Shanghai JiaoTong University School of Medicine, 200011, Shanghai, China

    Bin Chen

  6. Ear Institute, Shanghai JiaoTong University, 200011, Shanghai, China

    Bin Chen

  7. Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, 200011, Shanghai, China

    Bin Chen

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  1. Feng Wang
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  2. Wen Jiang
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Contributions

Conception and design of the research: FW and WJ; acquisition of data: FW and WJ; analysis and interpretation of data: RL and BC; statistical analysis: RL and BC; drafting the manuscript: FW and WJ; revision of manuscript for important intellectual content: RL and BC. All authors have read and approved the final manuscript FW and WJ contributed equally to this work.

Corresponding authors

Correspondence to Bin Chen or Rongrong Li.

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COMPLIANCE WITH ETHICAL STANDARDS

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

AVAILABILITY OF DATA AND MATERIAL

The data used to support the findings of this study are available from the corresponding author upon request.

ADDITION INFORMATION

Feng Wang and Wen Jiang contributed equally to this work.

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Feng Wang, Jiang, W., Chen, B. et al. The Mechanism of MSX1 and PAX9 Implication in Tooth Development Based on the Weighted Gene Co-Expression Network Analysis. Russ J Dev Biol 52, 187–198 (2021). https://doi.org/10.1134/S1062360421030085

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