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The Role of EGCG on the Expression of p38 MAPK, IL-6 and TNF-α Biomarker Proteins in the Cisplatin Induced Kidney Damage in Rats

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Abstract

EGCG, a polyphenolic catechin, has anticancer, antiinflammatory and antioxidant effects on kidney tissue. It was aimed to determine the antiinflammatory, antioxidant effect of EGCG against cisplatin-induced nephrotoxicity. 28 male Wistar albino (n = 28, 8 weeks old) rats were used. Rats were divided into 4 groups and each group included 7 rats. The groups were: (i) Control Group: fed with a standard diet; (ii) EGCG Group: Standard diet + EGCG; (iii) Cis Group: Standard diet + Cis; (iv) Cis + EGCG Group: They were fed with standard diet + Cis + EGCG. In the EGCG-treated group (Cis + EGCG), the expression levels of p38α MAPK, IL-6 and TNF-α proteins, which are inflammation markers, were found to be significantly decreased compared to the Cis group (p < 0.05). Our results show that cisplatin increases MDA levels from antioxidant defense systems, decreases glutathione (GSH) and catalase (CAT) activity, thus activating oxidative metabolism and causing damage. In the histopathological examination, it was observed that kidney damage occurred significantly in the cisplatin-administered group, whereas it decreased in the EGCG-administered group. These results show that EGCG treatment has an antiinflammatory effect and it can significantly prevent nephrotoxicity.

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Funding

This study was supported by the Fırat University Scientific Research Projects Coordination Unit (FUBAP) with the project number FF.20.04.

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Authors and Affiliations

  1. Department of Biology, Molecular Biology and Genetics Program, Faculty of Science, Firat University, Elazig, Turkey

    Nuran Toprakoglu, Abdullah Aslan, Seda Beyaz & Ozlem Gok

  2. Department of Biology, Faculty of Science, Firat University, Elazig, Turkey

    Orhan Erman

  3. Department of Physiology, Faculty of Medicine, Firat University, Elazig, Turkey

    Fatih Tan

  4. Department of Pathology, Medical School, Firat University, Elazig, Turkey

    Ibrahim Hanifi Ozercan

  5. Faculty of Health Sciences, Firat University, Elazig, Turkey

    Fazilet Erman

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  1. Nuran Toprakoglu
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  2. Abdullah Aslan
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Contributions

AA: wrote the article, review-editing, NT, SB, OG, FT, FE: laboratuary analysis, reading article, formal analysis, OE: reading article, IHO: histopathological analysis, formal analysis.

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Correspondence to Abdullah Aslan.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. All of the animal experimentation work of this study was made at the Firat University Experimental Animals Research Institute (FUDAM), meeting date November 27, 2019, protocol number 2019-141, meeting number 2019-22, decision number: 222.

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Nuran Toprakoglu, Aslan, A., Beyaz, S. et al. The Role of EGCG on the Expression of p38 MAPK, IL-6 and TNF-α Biomarker Proteins in the Cisplatin Induced Kidney Damage in Rats. Biol Bull Russ Acad Sci 50, 555–565 (2023). https://doi.org/10.1134/S1062359022602336

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  • DOI: https://doi.org/10.1134/S1062359022602336

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