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Study on the Antioxidant Effects of Suaeda rigida Polysaccharides In Vitro and In Vivo

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Abstract

The objectives of this study were to investigate the antioxidant effects of Suaeda rigida polysaccharide (SRPs) in vitro and in vivo. Three free radical scavenging experiments, including DPPH, •OH and \({\text{O}}_{2}^{{\centerdot - }}\) were used to study the antioxidant effects of the SRPs in vitro. The antioxidant effects of three concentrations of the SRPs on the immunosuppressive mice were analyzed by UV-Vis spectrophotometry and chemical colorimetry. The study is mainly from the aspects of the NOS-NO-cGMP signaling pathway, enzymatic and non-enzymatic antioxidant systems, and immune organ index. The results showed that the SRPs could effectively scavenge the DPPH, •OH and \({\text{O}}_{2}^{{\centerdot - }}\) in vitro, and the scavenging rate increased with the increase of its concentration in a specific range. In addition, the SRPs could significantly affect the NOS-NO-cGMP signaling pathway, enzymatic and non-enzymatic antioxidant systems (CAT, GSH-Px, GST, GSH, etc.), and immune organ index in the immunosuppressive mice, so as to exert the antioxidant effects. All the results suggested that the SRPs had significant antioxidant effects in vitro and in vivo. The mechanisms were related to scavenging the DPPH, •OH and \({\text{O}}_{2}^{{\centerdot - }}\) in vitro, reducing the contents of •OH, MDA, H2O2, increasing the GSH content, enhancing the activities of CAT, GSH-Px, GST, and weakening the XOD activity, finally inhibiting the NOS-NO-cGMP signaling pathway in the immunosuppressed mice.

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ACKNOWLEDGMENTS

The authors are pleased to acknowledge the experimental station, School of Animal Sciences, Tarim University. The authors are very grateful to Chenchen Chang, Zhengkun Xu, Shiyao Zhang and Jun Pang (Tarim University, Alar, Xinjiang, China).

Funding

This study was supported by the Science and technology research project of Chongqing Education Commission (KJQN201901235, KJQN20201226, KJQN202101247).

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Authors and Affiliations

  1. School of Teacher Education, Chongqing Three Gorges University, 404120, Chongqing, China

    Ailong Sha

  2. School of Biology and Food Engineering, Chongqing Three Gorges University, 404120, Chongqing, China

    Hongrun Chen

  3. School of Environmental and Chemical Engineering, Chongqing Three Gorges University, 404120, Chongqing, China

    Haiyan Hao

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  1. Ailong Sha
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  2. Hongrun Chen
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Correspondence to Ailong Sha.

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Conflict of interest. The authors have declared that there were no conflicts of interest.

Statement on the welfare of animals. The care and use of mice were approved by the Scientific Ethics Committee for Experiments on Animals of the Tarim University. All applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Ailong Sha, Chen, H. & Hao, H. Study on the Antioxidant Effects of Suaeda rigida Polysaccharides In Vitro and In Vivo. Biol Bull Russ Acad Sci 50, 250–258 (2023). https://doi.org/10.1134/S1062359022602282

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  • DOI: https://doi.org/10.1134/S1062359022602282

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