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Study of the Application of Sprague-Dawley Rats for Disease Research Based on Hematological Parameters

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Abstract

This work aimed to obtain hematological parameters of Sprague-Dawley (SD) rats with different ages and genders, which supports the application of SD in drug screening, disease diagnosis, and treatment research. 26 hematological parameters of SD rats with ages of 3-12-21-week-old were obtained, 12-week-old were the least discrete, 3-week-old were obviously clustered together. White blood cell count (WBC), Mononuclear cell count (MONO), Platelet (PLT) and Platelet count (PCT) were significantly discrete at all weekly ages, while Mean corpuscular hemoglobin concentration (MCHC), High fluorescence intensity reticulocyte ratio (HFR), and Red blood cell distribution width-CV value (RDW-CV) were relatively clustered. The LYMPH (%), HFR (%), HCT (%), MCV, NEUT, MONO, RET (%), RDW-CV (%), and MCHC have a significance in different ages, as well as HCT (%), RBC, RDW-CV (%), RDW-SD in different genders. In conclusion, 12 weeks old SD rats should be selected as far as possible when testing hematological parameters, and 3 weeks old rats should be used only for adolescent related diseases research; gender is a great important factor in hematological parameters; MCHC, HFR and RDW-CV may be a marker in disease model of SD rat. These data will provide useful clinical indices for disease research of SD rat.

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Funding

This study was funded by the Scientific Research Fund of Education Department for Yunnan (2018JS89), Yunnan Applied Basic Research Projects for Kunming Medicine University special fund (2017FE468(-134)), and The Hundred-Talent Program of Kunming Medical University. A grant (no. 2019KF003) from State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University.

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Authors and Affiliations

  1. Department of Laboratory Animal Science, Kunming Medical University, Kunming, Yunnan, China

    Limei Wang & Xue Cao

  2. Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan, China

    Huixiang Zhang

  3. School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan, China

    Renhua Yang

  4. School of Basic Medical Sciences, Kunming Medical University, Kunming, Yunnan, China

    Yonghua Luo

  5. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan, China

    Xue Cao

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  1. Limei Wang
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  3. Renhua Yang
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Correspondence to Xue Cao.

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Conflict of interest. Authors have received research grants from Kunming Medical University. They declare that they have no conflicts of interest.

Statement on the welfare of animals. This study was following the guidelines of by-laws on laboratory animals, and was approved by the Ethics and Experimental Animal Committee of Kunming Medical University, China (KMMU2018028).

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Limei Wang and Huixiang Zhang contributed equally to this work.

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Limei Wang, Zhang, H., Yang, R. et al. Study of the Application of Sprague-Dawley Rats for Disease Research Based on Hematological Parameters. Biol Bull Russ Acad Sci 49, 392–399 (2022). https://doi.org/10.1134/S1062359022050120

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