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Effect of Cell Passage Time on the Electrotransfection Efficiency

  • CELL BIOLOGY
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Abstract—

Gene electrotransfer is an effective and promising gene delivery technique in clinical applications, such as DNA vaccination and gene therapy. An improved gene therapy protocol depends on the the proper establishment of the gene transfer method. Electroporation has been widely employed in in vitro and in vivo protocols, and increaing its transfection efficiency has been the field of research. In order to achieve the the maximal introduction of plasmid DNA into cells with optimal cell viability, electro transfection conditions for every single cell type should be determined individually. In this work, the effect of cell passage time on the electrotransfection efficiency of CHO cells is determined. The selected cell passage times of 24 and 48 h prior to the electroporation are considered for the analysis. It is shown that electrotransfection efficiency with all plasmid concentrations significantly differs when comparing 24 and 48 h cell passage times. However, only slight change in the cell viability is observed at 24 and 48 h of cell passage times.

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ACKNOWLEDGMENTS

We are grateful to Milda Jakutaviciute for assistance in language editing.

Funding

This research is funded by the European Social Fund according to the activity “Improvement of Researchers’’ Qualification by Implementing World-Class R&D Projects” of Measure no. 09.3.3-LMT-K-712-01-0188.

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  1. Biophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos str. 8, LT 44404, Kaunas, Lithuania

    Sonam Chopra, Paulius Ruzgys, Martynas Maciulevičius & Saulius Šatkauskas

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  1. Sonam Chopra
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  2. Paulius Ruzgys
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Correspondence to Sonam Chopra, Paulius Ruzgys, Martynas Maciulevičius or Saulius Šatkauskas.

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Sonam Chopra, Ruzgys, P., Maciulevičius, M. et al. Effect of Cell Passage Time on the Electrotransfection Efficiency. Biol Bull Russ Acad Sci 47, 441–447 (2020). https://doi.org/10.1134/S1062359020550014

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