Abstract
A novel liquid chromatographic method for the measurement of essential oils within animal feed additives was developed and applied. The simple and effective extraction protocol described yielded analyte recoveries more than 90% for cinnamaldehyde, eugenol, carvacrol, capsaicin, and dihydrocapsaicin for the feed additives tested. These five analytes were separated with a peak resolution of more than 1 on a C-18 reversed phase column yielding between 500 000–2 000 000 theoretical plates per meter. UV absorption detection at 210 nm provided limits of detection of approximately 1 µg of analyte per gram of solution for all analytes. Limits of quantitation were approximately 4 µg of analyte per gram of solution. Results indicated that while capsaicin and dihydrocapsaicin were present at conserved levels within feed additives tested (0.2 and 0.05%, respectively), the content of cinnamaldehyde, eugenol, and carvacrol varied considerably. The new method will find use within the emerging field of targeted nutrition for increasing gains for ruminants and poultry.
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REFERENCES
Valenzuela-Grijalva, N.V., Pinelli-Saavedra, A., Muhlia-Almazan, A., Domínguez-Díaz, D., and González-Ríos, H., J. Anim. Sci. Technol., 2017, vol. 59, no. 1, p. 8. https://doi.org/10.1186/S40781-017-0133-9
Stevanović, Z.D., Bošnjak-Neumüller, J., Pajić-Lijaković, I., Raj J., and Vasiljević, M., Molecules, 2018, vol. 23, no. 7, p. 1717. https://doi.org/10.3390/molecules23071717
Aziz, Z.A.A., Ahmad, A., Setapar, S.H.M., Karakucuk, A., Azim, M.M., Lokhat, D., Rafatullah, M., Ganash, M., Kamal, M.A., and Ashraf, G.M., Curr. Drug Metab., 2018, vol. 19, no. 13, p. 1100. https://doi.org/10.2174/1389200219666180723144850
Tongnuanchan, P. and Benjakul, S., J. Food Sci., 2014, vol. 79, no. 7, p. R1231. https://doi.org/10.1111/1750-3841.12492
Wallace, R.J., Proc. Nutr. Soc., 2004, vol. 63, no. 4, p. 621. https://doi.org/10.1079/PNS2004393
Carrazco, A.V., Peterson, C.B., Zhao, Y., Pan, Y., McGlone, J.J., Depeters, E.J., and Mitloehner, F.M., Sustainability, 2020, vol. 12, no. 24, p. 10347. https://doi.org/10.3390/SU122410347
Stevanović, Z.D., Bošnjak-Neumüller, J., Pajić-Lijaković, I., Raj, J., and Vasiljević, M., Molecules, 2018, vol. 23, no. 7, p. 1717. https://doi.org/10.3390/molecules23071717
Calsamiglia, S., Busquet, M., Cardozo, P.W., Castillejos, L., and Ferret, A., J. Dairy Sci., 2007, vol. 90, no. 6, p. 2580. https://doi.org/10.3168/JDS.2006-644
Patra, A.K. and Yu, Z., Appl. Environ. Microbiol., 2012, vol. 78, no. 12, p. 4271. https://doi.org/10.1128/AEM.00309-12
Ulanowska, M. and Olas, B., Int. J. Mol. Sci., 2021, vol. 22, no. 7, p. 3671. https://doi.org/10.3390/IJMS22073671
Saeed, M., Khan, M.S., Alagawany, M., Farag, M.R., Alqaisi, O., Aqib, A.I., Qumar, M., Siddique, F., and Ramadan, M.F., Rend. Lincei., 2021, vol. 32, no. 2, p. 273. https://doi.org/10.1007/S12210-021-00985-3/tables/3
Peter, K.V., Handbook of Herbs and Spices, Sawston: Woodhead, 2012.
Cardozo, P.W., Calsamiglia, S., Ferret, A., and Kamel, C., J. Anim. Sci., 2004, vol. 82, no. 11, p. 3230. https://doi.org/10.2527/2004.82113230X
Busquet, M., Calsamiglia, S., Ferret, A., Cardozo, P.W., and Kamel, C., J. Dairy Sci., 2005, vol. 88, no. 7, p. 2508. https://doi.org/10.3168/jds.S0022-0302(05)72928-3
Ferme, D., Banjac, M., Calsamiglia, S., Busquet, M., Kamel, C., and Avguštin, G., Folia Microbiol., 2004, vol. 49, no. 2, p. 151. https://doi.org/10.1007/bf02931391
Benchaar, C., Animal, 2015, vol. 10, no. 3, p. 418. https://doi.org/10.1017/S175173111500230X
Kolling, G.J., Stivanin, S.C.B., Gabbi, A.M., Machado, F.S., Ferreira, A.L., Campos, M.M., Tomich, T.R., Cunha, C.S., Dill, S.W., Pereira, L.G.R., and Fischer, V., J. Dairy Sci., 2018, vol. 101, no. 5, p. 4221. https://doi.org/10.3168/jds.2017-13841
Olijhoek, D.W., Hellwing, A.L.F., Grevsen, K., Haveman, L.S., Chowdhury, M.R., Løvendahl P., Weisbjerg, M.R., Noel, S.J., Højberg, O., Wiking, L., and Lund, P., J. Dairy Sci., 2019, vol. 102, no. 11, p. 9902. https://doi.org/10.3168/jds.2019-16329
Benchaar, C., J. Dairy Sci., 2020, vol. 103, no. 2, p. 1516. https://doi.org/10.3168/JDS.2019-17230
Nostro, A. and Papalia, T., Recent Pat. Antiinfect. Drug Discovery, 2012, vol. 7, no. 1, p. 28. https://doi.org/10.2174/157489112799829684
Marchese, A., Arciola, C.R., Coppo, E., Barbieri, R., Barreca, D., Chebaibi, S., Sobarzo-Sánchez, E., Nabavi, S.F., Nabavi, S.M., and Daglia, M., Biofouling, 2018, vol. 34, no. 6, p. 630. https://doi.org/10.1080/08927014.2018.1480756
Rodríguez-Prado, M., Ferret, A., Zwieten, J., Gonzalez, L., Bravo, D., and Calsamiglia, S., J. Anim. Sci., 2012, vol. 90, no. 6, p. 1879. https://doi.org/10.2527/jas.2010-3191
Moraes, D.C.A., Nagi, J.G., Fritzen, J., Vitagliano, L.A., Oliveira, E.R., Oba, A., and Silva, C.A., Trop. Anim. Health Prod., 2022, vol. 54, no. 4, p. 241. https://doi.org/10.1007/S11250-022-03233-4
An, X., Wang, Y., Wang, R., Hao, X., Hu, Y., Guo, T., Zhang, J., Wang, W., Shi, X., Han, S., and Qi, J., Livest. Sci., 2020, vol. 234, p. 103982. https://doi.org/10.1016/j.livsci.2020.103982
Dai, Z., Sensors, 2023, vol. 23, no. 6, p. 3140. https://doi.org/10.3390/S23063140
Cao, T. and Thompson, J.E., PLoS One, 2014, vol. 9, no. 1, p. 84119. https://doi.org/10.1371/journal.pone.0084119
Glenn, K., He, J, Rochlin, R., Teng, S., Hecker, J.G., and Novosselov, I., Sci. Rep., 2023, vol. 13, no. 1, p. 3992. https://doi.org/10.1038/s41598-023-30778-7
Cao, T. and Thompson, J.E., Anal. Lett., 2017, vol. 50, no. 4, p. 712. https://doi.org/10.1080/00032719.2016.1190736
Cao, T., Zhang, Q., and Thompson J.E., J. Chem. Educ., 2015, vol. 92, no. 1, p. 106. https://doi.org/10.1021/ed500509p
Amaral, M.S.S., Hearn, M., and Marriott, P.J., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci.2022, vol. 1209, p. 123412. https://doi.org/10.1016/j.jchromb.2022.123412
Slougui, N., Slama, M., Boudaoud, Y., and Bendrihem, A.S., J. Chem., 2022, vol. 2022, p. 5880539. https://doi.org/10.1155/2022/5880539
Nickavar, B., Mojab, F., and Dolat-Abadi, R., Food Chem., 2005, vol. 90, no. 4, p. 609. https://doi.org/10.1016/j.foodchem.2004.04.020
Trendafilova, A., Todorova, M., Ivanova, V., Zhelev, P., and Aneva, I., Chem. Biodivers., 2021, vol. 18, no. 10, p. e2100498. https://doi.org/10.1002/cbdv.202100498
Karunasekara, T. and Poole, C.F., J. Chromatogr. A, 2012, vol. 1235, p. 159. https://doi.org/10.1016/j.chroma.2012.02.043
Venskutonis, P.R., Dapkevicius, A., and Baranauskiene, M., J. Essent. Oil Res., 1997, vol. 9, no. 1, p. 107. https://doi.org/10.1080/10412905.1997.9700727
Aburjai, T., Hu, M., and Cavrini, V., J. Essent. Oil Res., 2011, vol. 17, no. 1, p. 49. https://doi.org/10.1080/10412905.2005.9698827
Cao, C., Liu, W., Babajanian, S., Zhang, Y., Chang, P., and Swanson, G., J. AOAC Int., 2020, vol. 103, no. 5, p. 1394. https://doi.org/10.1093/jaoacint/qsaa031
Lopes, J.D.S., Lima, A.B.S. de, Cangussu, R.R. da, Silva, M.V. da, Ferrão, S.P.B., and Santos, L.S., Food Chem., 2022, vol. 368, p. 130746. https://doi.org/10.1016/j.foodchem.2021.130746
Pages-Rebull, J., Pérez-Ràfols, C., Serrano, N., del Valle, M., and Díaz-Cruz, J.M., Food Biosci., 2023, vol. 52, p. 102401. https://doi.org/10.1016/j.fbio.2023.102401
Larcher, R., Nicolini, G., Puecher, C., Bertoldi, D., Moser, S., and Favaro, G., Anal. Chim. Acta, 2007, vol. 582, no. 1, p. 55. https://doi.org/10.1016/j.aca.2006.08.056
de Jager, L.S., Perfetti, G.A., and Diachenko, G.W., J. Chromatogr. A, 2007, vol. 1145, nos. 1–2, p. 83. https://doi.org/10.1016/j.chroma.2007.01.039
Villa, C., Gambaro, R., Mariani, E., and Dorato, S., J. Pharm. Biomed. Anal., 2007, vol. 44, no. 3, p. 755. https://doi.org/10.1016/J.JPBA.2007.03.020
Viñas, P., Soler-Romera, M.J., and Hernández-Córdoba, M., Talanta, 2006, vol. 69, no. 5, p. 1063. https://doi.org/10.1016/j.talanta.2005.12.030
Schwarz, K., Ernst, H., and Ternes, W., J. Sci. Food Agric., vol. 70, no. 2, p. 217. https://doi.org/10.1002/(SICI)1097-0010(199602)70:2<217::AID-JSFA488>3.0.CO;2-Y
Cantalapiedra, A., Gismera, M.J., Sevilla, M.T., and Procopio, J.R., Phytochem. Anal., 2014, vol. 25, no. 3, p. 247. https://doi.org/10.1002/PCA.2500
Yazdanpanah, E., Int. J. Pharm. Technol., 2016, vol. 8, no. 3, p. 16045.
Pages-Rebull, J., Pérez-Ràfols, C., Serrano, N., del Valle, M., and Díaz-Cruz, J.M., Food Biosci., 2023, vol. 52, p. 102401. https://doi.org/10.1016/j.fboio.2023.102401
Jaicharoensub, J., Sakpakdeejaroen, I., and Panthong, S., Talanta Open, 2023, vol. 7, p. 100227. https://doi.org/10.1016/j.talo.2023.100227
Sandell, E.B. and Meehan, E.J., J. Chem. Educ., 2004, vol. 81, no. 12, p. 1715. https://doi.org/10.1021/ed081P1715.2
Thompson, J.E. and Brode, L., J. Chem. Educ., 2016, vol. 93, no. 6, p. 988. https://doi.org/10.1021/acs.jchemed.5b00773
Theoretical Plate Number and Symmetry Factor : SHIMADZU (Shimadzu Corporation). https://www.shimadzu.com/an/service-support/technical-support/analysis-basics/basic/theoretical_plate.html. Accessed April 23, 2023.
Harris, D.C. and Lucy, C.A., Quantitative Chemical Analysis, New York: Macmillan, 2020, 10th ed.
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Diab, H., Thompson, J. A New Liquid Chromatography Method for the Determination of Essential Oils in Animal Feed Additives. J Anal Chem 79, 191–199 (2024). https://doi.org/10.1134/S1061934824020059
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DOI: https://doi.org/10.1134/S1061934824020059