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Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine

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Abstract

A method is proposed for the voltammetric determination of measles antibodies using a glassy carbon electrode modified with 2-propargylthio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. An analytical signal was generated using a synthesized original compound attached to the surface of carboxylated carbon nanotubes through a thioline reaction and acted as a cross-linker for the immobilization of a bioreceptor, the measles virus antigen. We selected optimal working conditions for determining measles antibodies: signal recording mode, incubation time, temperature, pH of measurements, and concentration of the immobilized antigen. A calibration dependence I* (%) = –(1.5 ± 0.7)log с(IU/mL) + (29 ± 2) (R2 = 0.9653) was linear in the concentration range of measles antibodies 0.1–5.0 IU/mL. The limit of detection equal to 0.17 IU/mL, calculated using the 3σ test, ensures the use of the proposed method for the rapid analysis of biological fluids.

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Funding

This work was supported by the Russian Science Foundation, project no. 20-13-00142-P.

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Authors and Affiliations

  1. Ural Federal University, 620002, Yekaterinburg, Russia

    M. V. Medvedeva, A. V. Mazur, T. S. Svalova, I. A. Balin, V. L. Rusinov, A. I. Matern & A. N. Kozitsina

  2. Postovskii Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences, 620108, Yekaterinburg, Russia

    V. L. Rusinov

Authors
  1. M. V. Medvedeva
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  2. A. V. Mazur
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  3. T. S. Svalova
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  4. I. A. Balin
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  5. V. L. Rusinov
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  6. A. I. Matern
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  7. A. N. Kozitsina
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Correspondence to M. V. Medvedeva or T. S. Svalova.

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Translated by O. Zhukova

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Medvedeva, M.V., Mazur, A.V., Svalova, T.S. et al. Voltammetric Determination of Measles Virus Antibodies Using a Glassy Carbon Electrode Modified with 2-Propargyltio-6-nitro-7-hydroxy-4H-1,2,4-triazolo-4,7-dihydro[5,1-c]-1,2,4-triazine. J Anal Chem 78, 1694–1700 (2023). https://doi.org/10.1134/S1061934823120109

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