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Green Flow Injection Spectrophotometry with Betel Nut (Areca catechu L.) Natural Reagent for Indirect Determination of Ascorbic Acid

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Abstract

A green flow injection spectrophotometric technique for the determination of ascorbic acid was described based on the redox and complexation reactions. Iron(III) was reduced to iron(II) by ascorbic acid with subsequent complexation reaction of remaining iron(III) with a natural reagent extracted from Areca catechu L. at pH 5.0 yielding a dark-blue color. Absorbance decreased with increasing ascorbic acid concentration. A linear range of 1–10 mg/L for ascorbic acid was obtained with r2 of 0.9986. Limit of detection and limit of quantification were 0.3 and 0.9 mg/L, respectively. Relative standard deviation was less than 5.0%. This method was utilized to quantify ascorbic acid in pharmaceuticals and soft drinks with a sampling rate of 40 h−1. Ascorbic acid contents of 96–100% in pharmaceutical samples were obtained, while recoveries for spiked ascorbic acid standard at 3 mg/L in soft drinks were 92–113%.

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ACKNOWLEDGMENTS

We would like to thank the Department of Chemistry, Faculty of Science, Mahasarakham University for providing laboratory facilities. Supharoek S.-a. acknowledges Mahidol University, Amnatcharoen Campus.

Funding

This research project was financially supported by Thailand Science Research and Innovation (TSRI) 2021. Additional financial support was received from the Faculty of Science, Mahasarakham University, and the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation.

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Authors and Affiliations

  1. Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University, 10400, Bangkok, Thailand

    Sam-ang Supharoek

  2. Department of Medical Science, Amnatcharoen Campus, Mahidol University, 37000, Amnat Charoen, Thailand

    Sam-ang Supharoek

  3. Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, 44150, Maha Sarakham, Thailand

    Bordin Weerasuk & Kraingkrai Ponhong

  4. Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Analytical Science and Technology, Chiang Mai University, 50200, Chiang Mai, Thailand

    Kate Grudpan

Authors
  1. Sam-ang Supharoek
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  2. Bordin Weerasuk
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  4. Kraingkrai Ponhong
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Correspondence to Kraingkrai Ponhong.

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Supharoek, Sa., Weerasuk, B., Grudpan, K. et al. Green Flow Injection Spectrophotometry with Betel Nut (Areca catechu L.) Natural Reagent for Indirect Determination of Ascorbic Acid. J Anal Chem 77, 1274–1281 (2022). https://doi.org/10.1134/S1061934822100148

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  • DOI: https://doi.org/10.1134/S1061934822100148

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