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New approaches to the calculation and interpretation of asymmetry factors of chromatographic peaks

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Abstract

The suitability of the determination of the asymmetry factor of chromatographic peaks by the ratio of areas of two components separated by a perpendicular dropped from the maximum of the peak to the base-line, A s * = S b /S a , where symbol a corresponds to the leading edge of the peak and b is for its tailing slope, is discussed. It is demonstrated that this method enables the estimation of the asymmetry of even partially separated chromatographic signals, including those eluted “in the tail” of intense peaks of solvents. The concepts of the asymmetry index I(A s *) and its increment ΔI(A s *) = (A s *)–I(A s *) are introduced, which ensures the characterization of the asymmetry of peaks of polar analytes with respect to the asymmetry of nonpolar reference components, that is, the separation of the effects of the polarity of analytes and their quantities injected into the chromatographic column on this parameter. For the first time we revealed a correlation of the asymmetry factors of compounds of different chemical nature with such a characteristic of their polarity as the difference in chromatographic separation temperature and the normal boiling point of analytes.

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References

  1. Blumberg, L.M., Temperature-Programmed Gas Chromatography, Weinheim: Wiley, 2010.

    Book  Google Scholar 

  2. Encyclopedia of Chromatography, Cazes, J., Ed., Boca Raton, FL: CRC, 2010, 3rd ed.

  3. Dolan, J.W., LC–GC Europe, 2003, no. 9, p. 2.

    Google Scholar 

  4. Stolyarov, B.V., Savinov, I.M., Vitenberg, A.G., Kartsova, L.A., Zenkevich, I.G., Kalmanovskii, V.I., and Kalambet, Yu.A., Prakticheskaya gazovaya i zhidkostnaya khromatografiya (Practical Gas and Liquid Chromatography), St. Petersburg: St. Petersburg. Gos. Univ., 2002.

    Google Scholar 

  5. Gol’bert, K.A. and Vigdergauz, M.S., Kurs gazovoi khromatografii (Course of Gas Chromatography), Moscow: Khimiya, 1974.

    Google Scholar 

  6. Yashin, Ya.I., Fiziko-khimicheskie osnovy khromatograficheskogo razdeleniya (Physicochemical Fundamentals of Chromatographic Separation), Moscow: Khimiya, 1976.

    Google Scholar 

  7. Sakodynskii, K.I., Brazhnikov, V.V., Volkov, S.A., Zel’venskii, V.Yu., Gankina, E.S., and Shatts, V.D., Analiticheskaya khromatografiya (Analytical Chromatography), Moscow: Khimiya, 1993.

    Google Scholar 

  8. Ettre, L.S., LC–GC North America, 2003, vol. 21, no. 1, p. 12.

    CAS  Google Scholar 

  9. Jonsson, J.A., LC–GC North America, 2002, vol. 20, no. 10, p. 920.

    Google Scholar 

  10. European Pharmacopoeia, Council of Europe, 2004, 5th ed., 2014, 8th ed.

  11. Evaluating System Suitability CE, GC LC and A/D ChemStation, Revisions: A.03.0x-A.08.0x, Agilent Technologies. www.agilent.com/cs/library/Support/ Documents/a10424.pdf. Cited September, 2016.

  12. Hsu, J.T. and Chen, T.-L., J. Chromatogr. A, 1987, vol. 404, p. 1.

    Article  CAS  Google Scholar 

  13. Olive, J. and Grimalt, J.O., J. Chromatogr. Sci., 1991, vol. 29, no. 2, p. 70.

    Article  CAS  Google Scholar 

  14. Row, K.H. and Choi, D.-K., Sep. Sci. Technol., 1995, vol. 30, no. 19, p. 3615.

    Article  CAS  Google Scholar 

  15. Lan, K. and Jorgenson, J.W., J. Chromatogr. A, 2001, vol. 915, p. 1.

    Article  CAS  Google Scholar 

  16. Li, J., J. Chromatogr. A, 2002, vol. 952, p. 63.

    Article  CAS  Google Scholar 

  17. Papai, Z. and Pap, T.L., Analyst, 2002, vol. 127, p. 494.

    Article  CAS  Google Scholar 

  18. Papai, Z. and Pap, T.L., J. Chromatogr. A, 2002, vol. 953, p. 31.

    Article  CAS  Google Scholar 

  19. Pai, S.-C., J. Chromatogr. A, 2003, vol. 988, p. 233.

    Article  CAS  Google Scholar 

  20. Parks, M.L., Romero, L.A., and Whiting, J., Sandia Rep. SAN2005-4868, Sandia Nat. Lab., 2008.

    Google Scholar 

  21. Di Marco, V.B. and Bombi, G.G., J. Chromatogr. A, 2001, vol. 931, p. 1.

    Article  Google Scholar 

  22. Choi, D.Y. and Row, K.H., Biotechnol. Bioprocess Eng., 2004, vol. 9, p. 495.

    Article  CAS  Google Scholar 

  23. Hatik, S. and Hrouzek, J., Chem. Pap., 1994, vol. 48, no. 6, p. 376.

    Google Scholar 

  24. Yu, T. and Peng, H., Bioinformatics, 2010, vol. 11, p. 559.

    Google Scholar 

  25. Dolan, J.W., LC–GC, 1987, vol. 15, no. 11, p. 1.

    Google Scholar 

  26. Al-Bajjari, T.I., Le Vent, S., and Taylor, D.R., J. Chromatogr. A, 1994, vol. 683, p. 377.

    Article  CAS  Google Scholar 

  27. Schudel, J.V.H. and Guiochon, G., J. Chromatogr. A, 1988, vol. 457, p. 1.

    Article  CAS  Google Scholar 

  28. Formula for Calculating the Number of Theoretical Plates, Shimadzu Corp. http://www.shimadzu.com/an/hplc/support/lib/lctalk/34/34tec.html. Cited September, 2016.

  29. Gotmar, G., Fornstedt, T., and Guiochon, G., J. Chromatogr. A, 1999, vol. 831, p. 17.

    Article  CAS  Google Scholar 

  30. Williamson, Y. and Davis, J.M., Electrophoresis, 2006, vol. 27, p. 572.

    Article  CAS  Google Scholar 

  31. Jaulmes, A., Ignatiadis, I., Cardot, P., and Vidal-Madjar, C., J. Chromatogr. A, 1987, vol. 395, p. 291.

    Article  CAS  Google Scholar 

  32. Zenkevich, I.G. and Pavlovskii, A.A., J. Anal. Chem., 2015, vol. 70, no. 9, p. 1140.

    Article  Google Scholar 

  33. Zenkevich, I.G. and Pavlovskii, A.A., J. Sep. Sci., 2015, vol. 38, p. 2848.

    Article  CAS  Google Scholar 

  34. Makarov. A.A. and Zenkevich. I.G., Abstracts of Papers, Mezhd. konf. “Organicheskaya khimiya ot Butlerova i Bel’shteina do sovremennosti” (Int. Conf. on Organic Chemistry from Butlerov and Beilstein to Present), St. Petersburg, June 2006, no. 0-20, p. 50.

    Google Scholar 

  35. Makarov, A.A. and Zenkevich, I.G., Abstracts of Papers, 3rd Int. Symp. on Computer Application and Chemometrics in Analytical Chemistry, Lake Balaton, Hungary, July 2006, PL4.

    Google Scholar 

  36. Zenkevich, I.G. and Pavlovskii, A.A., Analitika Kontrol’, 2014, vol. 18, no. 2, p. 171.

    Google Scholar 

  37. Zenkevich, I.G. and Pavlovskii, A.A., Russ. J. Phys. Chem. A, 2016, vol. 90, no. 5, p. 1074.

    Article  CAS  Google Scholar 

  38. Pavlovskii, A.A., Heberger, K., and Zenkevich, I.G., J. Chromatogr. A, 2016, vol. 1445, p. 126.

    Article  CAS  Google Scholar 

  39. Heberger, K. and Zenkevich, I.G., J. Chromatogr. A, 2010, vol. 1217, p. 2895.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Chemistry, St. Petersburg State University, St. Petersburg, 198504, Russia

    I. G. Zenkevich, A. A. Makarov & A. A. Pavlovskii

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  1. I. G. Zenkevich
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  2. A. A. Makarov
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  3. A. A. Pavlovskii
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Correspondence to I. G. Zenkevich.

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Original Russian Text © I.G. Zenkevich, A.A. Makarov, A.A. Pavlovskii, 2017, published in Zhurnal Analiticheskoi Khimii, 2017, Vol. 72, No. 7, pp. 603–611.

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Zenkevich, I.G., Makarov, A.A. & Pavlovskii, A.A. New approaches to the calculation and interpretation of asymmetry factors of chromatographic peaks. J Anal Chem 72, 710–718 (2017). https://doi.org/10.1134/S1061934817070164

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  • DOI: https://doi.org/10.1134/S1061934817070164

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