Skip to main content
SpringerLink
Log in

Determination of protein adducts of organophosphorus nerve agents in blood plasma

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hank, E.D., Handbook of Chemical and Biological Warfare Agents, Boca Raton: CRC, 2008.

    Google Scholar 

  2. Degenhardt, C.E.A.M., Pleijsier, K., van der Schans, M.J., Langenberg J.P., Preston, K.E., Solano, M.I., Maggio, V.L., and Barr, J.R., J. Anal. Toxicol., 2004, vol. 28, p. 364.

    Article  CAS  Google Scholar 

  3. Kuca, K. and Patocka, J.R., J. Enzyme Inhib. Med. Chem., 2004, vol. 19, p. 39.

    Article  CAS  Google Scholar 

  4. Noort, D., Benschop, H.P., and Black, R., Toxicol. Appl. Pharmacol., 2002, vol. 184, p. 116.

    Article  CAS  Google Scholar 

  5. Abney, C.W., Knaack, J.L.S., Ali, A.A.I., and Johnson, R.C., Chem. Res. Toxicol., 2013, vol. 26, p. 775.

    Article  CAS  Google Scholar 

  6. Sporty, J.L.S., Lemire, S.W., Jakubowski, E.M., Renner, J.A., Evans, R.A., Williams, R.F., Schmidt, J.G., Van der Schans, M.J., Noort, D., and Johnson, R., Anal. Chem., 2010, vol. 82, p. 6593.

    Article  CAS  Google Scholar 

  7. Aryal, U.K., Lin, C.-T., Kim, J.-S., Heibeck, T.H., Wang, J., Qian, W.-J., and Lin, Y., Anal. Chim. Acta, 2012, vol. 723, p. 68.

    Article  CAS  Google Scholar 

  8. Worek, F., Thiermann, H., Szinicz, L., and Eyer, P., Biochem. Pharmacol., 2004, vol. 68, p. 2237.

    Article  CAS  Google Scholar 

  9. Aurbek, N., Thiermann, H., Szinicz, L., Eyer, P., and Worek, F., Toxicology, 2006, vol. 224, p. 91.

  10. Black, R.M., Harrison, J.M., and Read, R.W., Arch. Toxicol., 1999, vol. 73, p. 123.

    Article  CAS  Google Scholar 

  11. De Jong, L.P. and Van Dijk, C., Biochem. Pharmacol., 1984, vol. 33, no. 4, p. 663.

    Article  Google Scholar 

  12. Read, R.W., Riches, J.R., Stevens, J.A., Stubbs, S.J., and Black, R.M., Arch. Toxicol., 2010, vol. 84, no. 1, p. 25.

    Article  CAS  Google Scholar 

  13. Chen, S., Zhang, J., Lumley, L., and Cashman, J.R., J. Pharmacol. Exp. Ther., 2013, vol. 344, no. 2, p. 531.

    Article  CAS  Google Scholar 

  14. Bao, Y., Liu, Q., Chen, J., Lin, Y., Wu, B., and Xie, J., J. Chromatogr. A, 2012, vol. 1229, p. 164.

    Article  CAS  Google Scholar 

  15. Koryagina, N., Savelieva, E., Babakov, V., Dubrovskiy, Ya., Karakashev, G., Murashko, E., Ukolova, E., Kopeykin, V., Koneva, V., Khlebnikova, N., and Radilov, A., The Toxicologist: Supplement to Toxicological Sciences, 2015, no. 1, p. 144.

    Google Scholar 

  16. Koryagina, N.L., Savel’eva, E.I., Khlebnikova, N.S., Kopeikin, V.A., Koneva, V.Yu., and Radilov, A.S., Toksikol. Vestn., 2014, no. 4, p. 39.

    Google Scholar 

  17. Organisation for the Prohibition of Chemical Weapons. www.opcw.org/about-opcw/subsidiary-bodies/scientific-advisory-board/documents/reports/?tx_pagebrowse_pi1%5Bpage%5D=1&cHash=b42b0e05-e5164e555be95703d78d1dcc. Cited December 30, 2015.

  18. Koryagina, N.L., Savelieva, E.I., Kopeikin, V.A., Khlebnikova, N.S., Voitenko, N.G., Sorokoumov, P.V., Prokofieva, D.S., and Radilov, A.S., Abstracts of Papers, 16th Int. Chemical Weapons Demilitarization Conference (CWD) 2013, London, 2013, p. 28.

    Google Scholar 

  19. Carter, M.D., Crow, B.S., Pantazides, B.G., Watson, C.M., Thomas, J.D., Blake, T.A., and Johnson, R.C., Anal. Chem., 2013, vol. 85, no. 22, p. 11106.

    Article  CAS  Google Scholar 

  20. Jiang, W., Murashko, E.A., Dubrovskii, Y.A., Podolskaya, E.P., Babakov, V.N., Mikler, J., Nachon, F., Masson, P., Schopfer, L.M., and Lockridge, O., Anal. Biochem., 2013, vol. 439, p. 132.

    Article  CAS  Google Scholar 

  21. Murashko, E.A., Dubrovskii, Ya.A., Podol’skaya, E.P., and Babakov, V.N., Med. Akad. Zh., 2012, vol. 12, no. 3, p. 77.

    Google Scholar 

  22. Williams, N.H., Harrison, J.M., Read, R.W., and Black, R.M., Arch. Toxicol., 2004, vol. 81, p. 627.

    Article  Google Scholar 

  23. Lam, H., Deutsch, E.W., Eddes, J.S., Eng, J.K., King, N., Stein, S.E., and Aebersold, R., Proteomics, 2007, vol. 7, no. 5, p. 655.

    Article  CAS  Google Scholar 

  24. Podol’skaya, E.P. and Babakov, V.N., Nauchn. Priborostr., 2008, vol. 18, no. 4, p. 5.

    Google Scholar 

  25. Koryagina, N.L., Savel’eva, E.I., Khlebnikova, N.S., Ukolov, A.I., Ukolova, E.S., Karakashev, G.V., and Radilov, A.S., Mass-Spektrom., 2015, vol. 12, no. 4, p. 236.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical–Biological Agency of Russia, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663, Russia

    N. L. Koryagina, E. I. Savel’eva, G. V. Karakashev, V. N. Babakov, Ya. A. Dubrovskii, E. S. Ukolova, N. S. Khlebnikova, E. A. Murashko, V. Yu. Koneva, A. I. Ukolov, V. A. Kopeikin & A. S. Radilov

Authors
  1. N. L. Koryagina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. I. Savel’eva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. V. Karakashev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. N. Babakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ya. A. Dubrovskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. S. Ukolova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. N. S. Khlebnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. A. Murashko
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. V. Yu. Koneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. A. I. Ukolov
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. V. A. Kopeikin
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. A. S. Radilov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. L. Koryagina.

Additional information

Original Russian Text © N.L. Koryagina, E.I. Savel’eva, G.V. Karakashev, V.N. Babakov, Ya.A. Dubrovskii, E.S. Ukolova, N.S. Khlebnikova, E.A. Murashko, V.Yu. Koneva, A.I. Ukolov, V.A. Kopeikin, A.S. Radilov, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 8, pp. 883–893.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Koryagina, N.L., Savel’eva, E.I., Karakashev, G.V. et al. Determination of protein adducts of organophosphorus nerve agents in blood plasma. J Anal Chem 71, 849–859 (2016). https://doi.org/10.1134/S1061934816080086

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934816080086

Keywords

Search

Navigation