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An HPLC method for levofloxacin determination and its application in biomedical analysis

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Abstract

Levofloxacin is a bacteriostatic L-isomer of ofloxacin. This drug is a third generation fluoroquinolone. Its activity strongly depends on its concentration and the AUC/MIC ratio is the best indication of drug exposure. A simple, specific and fast HPLC method with fluorescence detection for assay of levofloxacin level in human plasma was developed. The separation was performed on a reversed phase column. The mobile phase contained 0.4% solution of triethylamine. The regression equation was linear within the concentration range 0.15–30.0 mg/L. The validation parameters i.e. accuracy, precision and stability did not exceed 10%. High recovery was observed. The developed method fulfils the validation parameters. Due to the short time of analysis it may be applied to biomedical analysis.

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Authors and Affiliations

  1. Department of Physical Pharmacy and Pharmacokinetics, Poznań University of Medical Sciences, Święcickiego 6 Street, 60-781, Poznań, Poland

    A. Czyrski

  2. Department of Clinical Pharmacy and Biopharmacy, Poznań University of Medical Sciences, Marii Magdaleny 14 Street, 61-861, Poznań, Poland

    E. Szałek

Authors
  1. A. Czyrski
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  2. E. Szałek
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Correspondence to A. Czyrski.

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Czyrski, A., Szałek, E. An HPLC method for levofloxacin determination and its application in biomedical analysis. J Anal Chem 71, 840–843 (2016). https://doi.org/10.1134/S1061934816080049

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  • DOI: https://doi.org/10.1134/S1061934816080049

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