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Chromatographic–mass spectrometric determination of free fatty acids in blood plasma and urine using extractive alkylation

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Abstract

A method is proposed for the extractive alkylation of free fatty acids (FFA) in blood plasma and urine for their subsequent chromatographic–mass spectrometric determination as methyl esters. The list of target compounds includes 19 saturated acids (C6–C24) and 20 unsaturated acids (C16–C24), including ω-3, ω-6, ω-9, and polyunsaturated fatty acids. For the simultaneous extraction of FFA from a matrix and derivatization, extractive alkylation with iodomethane was used. The peculiarity and advantage of the method is integration of the stages of extraction and preparation of volatile derivatives under mild conditions. The recovery and conversion are from 60 to 90%. The analytical range was from 0.02 to 20 µg/mL of a sample. The effect of samples storage conditions on the results of measurements is evaluated. The method was tested in the determination of FFA in blood plasma and urine of volunteers and laboratory animals.

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References

  1. Bicalha, B., David, F., Rumplel, K., Kindt, E., and Sandra, P., J. Chromatogr. A, 2008, vol. 1211, p. 120.

    Article  Google Scholar 

  2. Jones, P.M. and Bennett, M.J., Biochim. Biophys. Acta, 2011, vol. 1811, p. 657.

    Article  CAS  Google Scholar 

  3. Maltby, D.A. and Millington, D.S., Clin. Chim. Acta, 1986, vol. 155, p. 167.

    Article  CAS  Google Scholar 

  4. Yi, L., He, J., Liang, Y., Yuan, D., Gao, H., and Zhou, H., Chem. Phys. Lipids, 2007, vol. 150, p. 204.

    Article  CAS  Google Scholar 

  5. Han, L.D., Liang, Q.L., Wang, M., Hu, P., and Luo, G.A., Chin. Chem. Lett., 2009, vol. 20, p. 1103.

    Article  Google Scholar 

  6. Wei, G.L. and Zeng, E.Y., TrAC, Trends Anal. Chem., 2011, vol. 30, no. 9, p. 1429.

    Article  CAS  Google Scholar 

  7. Boden, G., Proc. Assoc. Am. Physicians, 1999, vol. 3, p. 241.

    Article  Google Scholar 

  8. Krylov, A.I., Khlebnikova, N.S., and Poluyaktova, S.K., Zh. Anal. Khim., 1991, vol. 46, no. 12, p. 2428.

    CAS  Google Scholar 

  9. Lisi, A.M., Trout, G.J., and Kazlauskas, R., J. Chromatogr. B: B: Biomed. Sci. Appl., 1991, vol. 563, p. 257.

    Article  CAS  Google Scholar 

  10. Lisi, A.M., Kazlauskas, R., and Trout, G.J., J. Chromatogr. B: B: Biomed. Sci. Appl., 1993, vol. 617, p. 265.

    Article  CAS  Google Scholar 

  11. Lisi, A.M., Kazlauskas, R., and Trout, G.J., J. Chromatogr. B: B: Biomed. Sci. Appl., 1997, vol. 692, p. 67.

    Article  CAS  Google Scholar 

  12. Pace-Asciak, C.R.J., Lipid Res., 1989, vol. 30, p. 451.

    CAS  Google Scholar 

  13. Brandstrom, A. and Tunggren, U., Acta Chem. Scand., 1969, vol. 23, no. 6, p. 2204.

    Article  CAS  Google Scholar 

  14. Bictasha, M., Ebbels, T.M., Chan, Q., Loo, R.L., Yapa, I.K.S., Brown, I.J., de Iorio, M., Daviglus, M.L., Holmes, E., Stamler, J., Nicholson, J.K., and Elliott, P., J. Clin. Epidemiol., 2010, vol. 6, p. 970.

    Article  Google Scholar 

  15. Teahan, O., Gamble, S., Holmes, E., Waxman, J., Nicholson, J.K., and Bevan, C., Anal. Chem., 2006, vol. 78, p. 4307.

    Article  CAS  Google Scholar 

  16. Pasikanti, K.K., Ho, P.C., and Chan, E.C., Rapid Commun. Mass Spectrom., 2008, vol. 22, p. 2984.

    Article  CAS  Google Scholar 

  17. Want, E.J., Cravatt, B.F., and Siuzdak, G., ChemBioChem., 2005, vol. 6, p. 1941.

    Article  CAS  Google Scholar 

  18. Dunn, W.B., Broadhurst, D., Ellis, D.I., Brown, M., Halsall, A., and O’Hagan, S., Int. J. Epidemiol., 2008, vol. 37, no. 1, p. 23.

    Article  Google Scholar 

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Authors and Affiliations

  1. Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Medical and Biological Agency, settlement Kuz’molovskii, railway station Kapitolovo, building 93, Leningradskaya oblast, 188663, Russia

    A. I. Ukolov, T. I. Orlova, E. I. Savel’eva & A. S. Radilov

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  1. A. I. Ukolov
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  2. T. I. Orlova
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  3. E. I. Savel’eva
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  4. A. S. Radilov
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Correspondence to A. I. Ukolov.

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Original Russian Text © A.I. Ukolov, T.I. Orlova, E.I. Savel’eva, A.S. Radilov, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 9, pp. 968–975.

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Ukolov, A.I., Orlova, T.I., Savel’eva, E.I. et al. Chromatographic–mass spectrometric determination of free fatty acids in blood plasma and urine using extractive alkylation. J Anal Chem 70, 1123–1130 (2015). https://doi.org/10.1134/S1061934815090178

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  • DOI: https://doi.org/10.1134/S1061934815090178

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