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Kinetic-spectrophotometric determination of neomycin

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Abstract

A new kinetically-based spectrophotometric method for the determination of micro quantities of neomycin is described in this paper. It is an adaptation of the modified Berthelot method which is a widely used colorimetric procedure for urea determination. The method was developed due to neomycin effect on 2,2′-dicarboxyindophenol formation. The experimental conditions for successful determination of neomycin were optimized. The relative standard deviation (RSD) for the neomycin concentration interval of 77–154 μg/mL, ranges from 0.7 to 1%. The method was directly applied to determining neomycin in ophthalmic drops. The obtained results in analyzed pharmaceutical samples were in accordance with disc-diffusion microbiological method results.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, P.O. Box 22418000, Niš, Serbia

    I. D. Rašić Mišić, G. Ž. Miletić, S. S. Mitić, D. A. Kostić & A. S. Djordjević

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  1. I. D. Rašić Mišić
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  2. G. Ž. Miletić
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  3. S. S. Mitić
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  4. D. A. Kostić
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  5. A. S. Djordjević
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Correspondence to I. D. Rašić Mišić.

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Rašić Mišić, I.D., Miletić, G.Ž., Mitić, S.S. et al. Kinetic-spectrophotometric determination of neomycin. J Anal Chem 70, 234–239 (2015). https://doi.org/10.1134/S106193481502015X

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